Genome-Wide Identification and Characterization of the CsFHY3/FAR1 Gene Family and Expression Analysis under Biotic and Abiotic Stresses in Tea Plants (Camellia sinensis)
Abstract
:1. Introduction
2. Results
2.1. Identification and Analysis of CsFHY3/FAR1 Genes
2.2. Phylogenetic Analysis of CsFHY3/FAR1s
2.3. Gene Structure and Motif Analysis
2.4. Analysis of Cis-Acting Elements in the Promoters of CsFHY3/FAR1
2.5. Protein Interaction Network of CsFHY3/FAR1 Proteins
2.6. Expression Analysis in the Different Tissues of Tea Plants
2.7. Expression Analysis of CsFHY3/FAR1s under Different Stresses
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Plant Materials and Stress Treatments
5.2. Identification of the CsFHY3/FAR1 Gene Family in Tea Plants
5.3. Sequence Analysis and Phylogenetic Tree Construction
5.4. Analysis of Gene Structure and Conserved Motifs
5.5. Prediction of the Cis-Acting Elements and Protein Interaction Network
5.6. Transcriptome Analysis
5.7. Quantitative Real-Time PCR (qRT-PCR) Analysis
5.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Gene ID | Amino Acids | MW (kDa) | pI | GRAVY | Instability Index | Aliphatic Index | Subcellular Location |
---|---|---|---|---|---|---|---|---|
CsFRS-1 | CSS002622.1 | 689 | 80.13 | 9.10 | −0.434 | 46.00 | 77.21 | chlo:4, vacu:3, cyto:2, nucl:1.5, cysk_nucl:1.5, mito:1, plas:1, extr:1 |
CsFRS-2 | CSS016876.1 | 757 | 86.03 | 6.20 | −0.318 | 47.61 | 77.64 | nucl:13, vacu:1 |
CsFRS-3 | CSS003256.1 | 769 | 87.81 | 5.79 | −0.509 | 53.55 | 72.65 | nucl:13, cyto:1 |
CsFRS-4 | CSS001271.1 | 588 | 68.71 | 8.91 | −0.560 | 48.43 | 68.59 | nucl:7, pero:2, cysk:1.5, cysk_plas:1.5, cyto:1, mito:1, vacu:1 |
CsFRS-5 | CSS001125.1 | 752 | 86.58 | 6.47 | −0.539 | 50.51 | 76.77 | nucl:13, vacu:1 |
CsFRS-6 | CSS013735.1 | 787 | 90.41 | 6.26 | −0.565 | 52.42 | 71.60 | nucl:14 |
CsFRS-7 | CSS042110.1 | 368 | 43.19 | 8.42 | −0.428 | 39.24 | 65.76 | cyto:7, cysk:5, nucl:1, golg:1 |
CsFRS-8 | CSS022313.1 | 702 | 80.33 | 9.28 | −0.385 | 41.50 | 80.24 | nucl:11, cyto:2, cysk:1 |
CsFRS-9 | CSS018104.1 | 691 | 80.13 | 6.04 | −0.351 | 42.06 | 77.54 | nucl:7, chlo:4, mito:2, cysk:1 |
CsFRS-10 | CSS039560.1 | 897 | 103.05 | 7.36 | −0.568 | 49.04 | 70.51 | nucl:13, plas:1 |
CsFRS-11 | CSS007312.1 | 690 | 79.47 | 9.06 | −0.436 | 39.05 | 70.04 | nucl:12, cyto:1, cysk:1 |
CsFRS-12 | CSS040860.1 | 846 | 97.07 | 6.98 | −0.687 | 52.72 | 63.43 | nucl:14 |
CsFRS-13 | CSS040864.1 | 767 | 88.40 | 7.38 | −0.692 | 57.85 | 66.75 | nucl:6, pero:4, chlo:2, cyto:1, mito_plas:1 |
CsFRS-14 | CSS035662.1 | 659 | 75.35 | 9.11 | −0.428 | 39.71 | 68.21 | chlo:9, nucl:4, vacu:1 |
CsFRS-15 | CSS052639.1 | 832 | 95.68 | 7.49 | −0.501 | 54.13 | 74.13 | nucl:13, vacu:1 |
CsFRS-16 | CSS022572.1 | 680 | 79.60 | 8.97 | −0.558 | 47.81 | 77.25 | cyto:8, nucl:4, mito:1, plas:1 |
CsFRS-17 | CSS003022.1 | 691 | 79.70 | 6.03 | −0.357 | 44.10 | 79.58 | nucl:6, mito:3.5, cyto_mito:2.5, chlo:2, cysk:2 |
CsFRS-18 | CSS008927.1 | 402 | 47.13 | 8.91 | −0.491 | 48.24 | 67.69 | cyto:8, nucl:2, plas:2, mito:1, pero:1 |
CsFRS-19 | CSS040724.1 | 848 | 96.73 | 8.70 | −0.525 | 45.91 | 70.06 | nucl:13, cyto:1 |
CsFRS-20 | CSS025022.1 | 680 | 79.13 | 6.56 | −0.594 | 55.50 | 68.50 | nucl:13, vacu:1 |
CsFRS-21 | CSS052868.1 | 727 | 83.96 | 8.33 | −0.583 | 35.12 | 64.68 | nucl:12, plas:1, vacu:1 |
CsFRS-22 | CSS032048.1 | 890 | 101.02 | 6.70 | −0.532 | 44.93 | 67.07 | nucl:12, cyto:2 |
CsFRS-23 | CSS025195.1 | 529 | 61.07 | 8.92 | −0.460 | 41.99 | 65.80 | chlo:11, nucl:2, mito:1 |
CsFRS-24 | CSS043153.1 | 799 | 90.80 | 6.04 | −0.467 | 47.21 | 72.60 | nucl:13, chlo:1 |
CsFRS-25 | CSS050875.1 | 616 | 71.73 | 6.65 | −0.501 | 47.28 | 76.25 | nucl:9, cyto:4, plas:1 |
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Liu, Z.; An, C.; Zhao, Y.; Xiao, Y.; Bao, L.; Gong, C.; Gao, Y. Genome-Wide Identification and Characterization of the CsFHY3/FAR1 Gene Family and Expression Analysis under Biotic and Abiotic Stresses in Tea Plants (Camellia sinensis). Plants 2021, 10, 570. https://doi.org/10.3390/plants10030570
Liu Z, An C, Zhao Y, Xiao Y, Bao L, Gong C, Gao Y. Genome-Wide Identification and Characterization of the CsFHY3/FAR1 Gene Family and Expression Analysis under Biotic and Abiotic Stresses in Tea Plants (Camellia sinensis). Plants. 2021; 10(3):570. https://doi.org/10.3390/plants10030570
Chicago/Turabian StyleLiu, Zhengjun, Chuanjing An, Yiqing Zhao, Yao Xiao, Lu Bao, Chunmei Gong, and Yuefang Gao. 2021. "Genome-Wide Identification and Characterization of the CsFHY3/FAR1 Gene Family and Expression Analysis under Biotic and Abiotic Stresses in Tea Plants (Camellia sinensis)" Plants 10, no. 3: 570. https://doi.org/10.3390/plants10030570