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Article

Vaccinium virgatum Aiton Leaves Extract Suppressed Lipid Accumulation and Uric Acid Production in 3T3-L1 Adipocytes

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Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan
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Faculty of Regional Innovation, University of Miyazaki, Miyazaki 889-2192, Japan
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Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki, Miyazaki 889-2192, Japan
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Department of Pharmaceutical Health Sciences, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, Nobeoka 882-8508, Japan
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Organization for Promotion of Tenure Track, University of Miyazaki, Miyazaki 889-2192, Japan
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Biolabo Co., Ltd., Hyogo, Kobe 650-0047, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Antonella Smeriglio
Plants 2021, 10(12), 2638; https://doi.org/10.3390/plants10122638
Received: 29 October 2021 / Revised: 26 November 2021 / Accepted: 27 November 2021 / Published: 30 November 2021
(This article belongs to the Special Issue Bioactivity of Blueberry Extracts)
Blueberry (Vaccinium virgatum Aiton; Kinisato 35 Gou) leaves have recently attracted increasing attention as a useful material for the prevention of lifestyle diseases. Here, we examined the effects of the hot water extract of blueberry leaves (BLEx) on lipogenesis and uric acid production in 3T3-L1 adipocytes. The results showed that BLEx suppressed lipid accumulation and the mRNA expression of differentiation markers in 3T3-L1 adipocytes. A fractionation study showed that the highly polymerized proanthocyanidin-rich fraction was responsible for this effect. Upon maturation to adipocytes, 3T3-L1 cells produced uric acid and tumor necrosis factor-α, and hypoxia stimulated the production of uric acid and xanthine oxidoreductase activity. BLEx suppressed the production of uric acid under these conditions. Although BLEx inhibited the enzymatic activity of xanthine oxidase, this activity was observed in several fractions containing catechin, epicatechin, chlorogenic acid, rutin, and low molecular weight proanthocyanidins. Taken together, these results indicate that BLEx contains various compounds with the ability to suppress lipid accumulation and uric acid production in adipocytes. View Full-Text
Keywords: adipocyte; differentiation; uric acid; xanthine oxidoreductase; blueberry leaf adipocyte; differentiation; uric acid; xanthine oxidoreductase; blueberry leaf
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MDPI and ACS Style

Yamasaki, M.; Kiue, Y.; Fujii, K.; Sushida, M.; Yamasaki, Y.; Sugamoto, K.; Suzuki, Y.; Koga, Y.; Kunitake, H.; Kai, H.; Ogawa, K.; Nishiyama, K.; Goto, Y.; Nakayama, T. Vaccinium virgatum Aiton Leaves Extract Suppressed Lipid Accumulation and Uric Acid Production in 3T3-L1 Adipocytes. Plants 2021, 10, 2638. https://doi.org/10.3390/plants10122638

AMA Style

Yamasaki M, Kiue Y, Fujii K, Sushida M, Yamasaki Y, Sugamoto K, Suzuki Y, Koga Y, Kunitake H, Kai H, Ogawa K, Nishiyama K, Goto Y, Nakayama T. Vaccinium virgatum Aiton Leaves Extract Suppressed Lipid Accumulation and Uric Acid Production in 3T3-L1 Adipocytes. Plants. 2021; 10(12):2638. https://doi.org/10.3390/plants10122638

Chicago/Turabian Style

Yamasaki, Masao, Yusei Kiue, Kento Fujii, Moe Sushida, Yumi Yamasaki, Kazuhiro Sugamoto, Yosuke Suzuki, Yasuko Koga, Hisato Kunitake, Hisahiro Kai, Kenjiro Ogawa, Kazuo Nishiyama, Yo Goto, and Takayuki Nakayama. 2021. "Vaccinium virgatum Aiton Leaves Extract Suppressed Lipid Accumulation and Uric Acid Production in 3T3-L1 Adipocytes" Plants 10, no. 12: 2638. https://doi.org/10.3390/plants10122638

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