Positive Salt Tolerance Modulation via Vermicompost Regulation of SOS1 Gene Expression and Antioxidant Homeostasis in Viciafaba Plant
Abstract
:1. Introduction
2. Results
2.1. Vermicompost Improves Morphological Traits in Broad Bean Plant
2.2. Vermicompost Maintains Element Homeostasis in Salt-Stressed Broad Bean Plant
2.3. Vermicompost Modulates Hormonal Status, Proline, Nitrogen and Protein Content in Broad Bean Plant
2.4. Vermicompost Reduces Lipid Peroxidation and H2O2 through the Improvement of Antioxidant Enzyme Activities in Broad Bean Plant
2.5. Vermicompost Modulate the Non-Enzymatic Antioxidantsin Broad Bean Plant
2.6. Hierarchical Clustering and PCA Analysis Show Positive Interactions between Treatments and Variables
2.7. Vermicompost Upregulates Gene Expression of Salt Overly Sensitive (SOS1) Gene in Broad Bean Plant
3. Discussion
4. Materials and Methods
4.1. Experimental Materials and Vermicompost Characterizations
4.2. Experimental Design
4.3. Measurement of Growth Traits
4.4. Biochemical Analysis
4.4.1. Elemental Analysis
4.4.2. Determination of Nitrogen
4.4.3. High-Performance Liquid Chromatographic (HPLC) Method for the Determination of Abscisic Acid and Indole Acetic Acid
4.4.4. Determination of Free Proline
4.4.5. Estimation of Protein Content
4.4.6. Determination of Endogenous MDA and H2O2 Content
4.4.7. Enzyme’s Extractions and Assays
- Estimation of catalase (EC 1.11.1.6) activity;
- Estimation of ascorbate peroxidase (APX, EC 1.11.1.11) activity
- Estimation of glutathione reductase (EC 1.6.4.2) activity
- Estimation of superoxide dismutase (SOD, EC 1.15.1.1) activity
- Estimation of poly phenol oxidase (PPO, EC 1.10.3.)
4.4.8. Non-Enzymatic Antioxidant Extractions and Assays
- Estimation of ascorbic acid content
- Estimation of glutathione content
- Extraction and determination of total phenolic content
4.5. Gene Expression Analysis of SOS1
4.6. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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STI of Parameters | S | 2.5% VC + S | 5% VC + S | 10% VC + S | 15% VC + S |
---|---|---|---|---|---|
PH | 0.33 bc ±0.06 | 0.31 c ± 0.03 | 0.38 bc ±0.03 | 0.55 a ± 0.08 | 0.45 ab ± 0.09 |
RFW | 0.31 b ± 0.10 | 0.30 b ± 0.08 | 0.54 a ± 0.07 | 0.62 a ± 0.01 | 0.51 a ± 0.09 |
SFW | 0.32 c ± 0.05 | 0.31 c ± 0.04 | 0.52 b ± 0.02 | 0.77 a ± 0.05 | 0.62 b ± 0.11 |
TFW | 0.32 c ± 0.06 | 0.31 c ± 0.05 | 0.53 b ± 0.02 | 0.72 a ± 0.03 | 0.58 b ± 0.06 |
RDW | 0.39 c ± 0.09 | 0.34 c ± 0.05 | 0.68 b ± 0.07 | 1.10 a ± 0.05 | 0.80 b ± 0.10 |
SDW | 0.36 c ± 0.07 | 0.37 c ± 0.08 | 0.59 b ± 0.12 | 0.81 a ± 0.06 | 0.61 b ± 0.04 |
TDW | 0.37 c ± 0.07 | 0.36 c ± 0.07 | 0.61 b ± 0.11 | 0.87 a ± 0.04 | 0.65 b ± 0.02 |
MFV of Parameters | S | 2.5% VC + S | 5% VC + S | 10% VC + S | 15% VC + S |
---|---|---|---|---|---|
PH | 18.5 bc ± 18.5 | 12.9 c ± 8.5 | 33.0 bc ± 8.1 | 80.5 a ± 23.7 | 53.4 ab ± 27.2 |
RFW | 26.1 b± 22.6 | 25.1 b ± 17.7 | 80.3 a ± 15.7 | 97.2 a ± 2.4 | 69.2 a ± 19.2 |
SFW | 9.8 c ± 8.1 | 7.2 c ± 7.2 | 45.5 b ± 4.3 | 89.7 a ± 9.1 | 62.4 b ± 20.0 |
TFW | 10.4 c ± 11.2 | 8.0 c ± 10.4 | 53.3 b ± 4.5 | 92.5 a ± 6.6 | 64.5 b ± 13.1 |
RDW | 11.2 c ± 10.8 | 5.1 c ± 6.1 | 45.3 b ± 7.8 | 94.7 a ± 5.6 | 59.6 b ± 11.9 |
SDW | 12.9 c ± 12.1 | 14.5 c ± 14.8 | 52.5 b ± 21.5 | 91.0 a ± 10.7 | 56.2 b ± 6.9 |
TDW | 12.5 c ± 12.1 | 11.4 c ± 12.2 | 51.9 b ± 17.8 | 94.8 a ± 6.3 | 58.9 b ± 2.5 |
MFV | 14.5 c ± 9.7 | 12.0 c ± 10.3 | 51.7 b ± 8.8 | 91.5 a ± 6.1 | 60.6 b ± 4.3 |
Ranking of MFV means | 3 | 3 | 2 | 1 | 2 |
Plant Tissues | Control | 2.5%VC | 10%VC | S | 2.5%VC + S | 10%VC + S | |
---|---|---|---|---|---|---|---|
Mg2+ (g 100 g−1 DW) | Roots | 0.82 ab ± 0.05 | 0.92 a ± 0.04 | 0.76 bc ± 0.06 | 0.60 d ± 0.11 | 0.65 cd ± 0.08 | 0.75 bc ± 0.11 |
Shoots | 0.36 ab ± 0.03 | 0.44 a ± 0.09 | 0.40 ab ± 0.05 | 0.22 c ± 0.05 | 0.25 c ± 0.06 | 0.30 bc ± 0.05 | |
K+/Na+ | Roots | 1.22 c ± 0.04 | 1.46 a ± 0.01 | 1.28 b ± 0.01 | 0.36 f ± 0.01 | 0.69 e ± 0.01 | 0.81 d ± 0.01 |
Shoots | 4.45 c ± 0.30 | 6.63 a ± 0.42 | 5.22 b ± 0.42 | 2.32 e ± 0.10 | 3.26 d ± 0.15 | 4.39 c ± 0.45 | |
Ca2+ (g 100 g−1 DW) | Roots | 1.81 e ± 0.10 | 2.37 bc ± 0.04 | 2.22 cd ± 0.05 | 2.10 d ± 0.11 | 2.51 b ± 0.13 | 2.96 a ± 0.09 |
Shoots | 1.07 e ± 0.04 | 1.29 cd ± 0.08 | 1.17 de ± 0.05 | 1.41 c ± 0.08 | 1.77 b ± 0.05 | 2.12 a ± 0.11 |
Parameters | Plant Tissues | Control | 2.5%VC | 10% VC | S | 2.5% VC + S | 10%VC + S |
---|---|---|---|---|---|---|---|
IAA (ng g−1 FW) | Roots | 246.1 c ± 5.37 | 252.8 bc ± 5.16 | 265.9 a ± 6.31 | 153.4 e ± 3.80 | 173.3 d ± 3.97 | 260.4 ab ± 0.0 |
Leaves | 176.4 b ± 11.17 | 188.9 b ± 8.11 | 247.6 a ± 6.71 | 95.2 d ± 3.72 | 136.1 c ± 6.44 | 240.1 a ± 8.53 | |
ABA (ng g−1 FW) | Roots | 22.65 e ± 5.31 | 29.50 e ± 5.31 | 54.40 d ± 5.31 | 324.7 a ± 5.31 | 281.6 b ± 5.31 | 82.04 c ± 5.31 |
Leaves | 60.86 e ± 3.89 | 85.75 d ± 3.89 | 84.34 d ± 3.89 | 294.1 a ± 3.89 | 160.7 b ± 3.89 | 113.8 c ± 3.89 | |
Proline (µg g−1 FW) | Roots | 0.15 d ± 0.02 | 0.16 d ± 0.02 | 0.34 b ± 0.01 | 0.64 a ± 0.03 | 0.38 b ± 0.02 | 0.26 c ± 0.03 |
Leaves | 0.14 d ± 0.03 | 0.19 d ± 0.09 | 0.40 c ± 0.04 | 0.85 a ± 0.02 | 0.54 b ± 0.04 | 0.36 c ± 0.01 | |
N (mg g−1 DW) | Roots | 27.17 a ± 0.72 | 27.27 a ± 0.67 | 26.64 a ± 1.67 | 21.54 b ± 0.49 | 22.12 b ± 0.95 | 23.27 b ± 0.53 |
Shoots | 27.07 a ± 0.94 | 28.92 a ± 1.91 | 27.46 a ± 0.53 | 22.25 c ± 0.72 | 22.93 bc ± 0.85 | 24.73 b ± 1.75 | |
Protein (mg g−1 DW) | Roots | 170.0 a ± 6.08 | 172.0 a ± 2.65 | 165.0 a ± 5.57 | 135.0 c ± 4.36 | 139.0 c ± 3.61 | 147.0 b ± 3.46 |
Shoots | 176.0 b ± 2.65 | 191.0 a ± 2.65 | 171.0 b ± 3.61 | 137.0 d ± 1.73 | 141.0 d ± 3.46 | 155.0 c ± 3.61 |
Parameters | Plant Tissues | Control | 2.5%VC | 10% VC | S | 2.5% VC + S | 10%VC + S |
---|---|---|---|---|---|---|---|
AA (nmol g−1 FW) | Roots | 10.10 b ± 0.62 | 11.20 b ± 1.91 | 12.70 b ± 1.14 | 44.80 a ± 1.41 | 46.20 a ± 2.60 | 48.0 a ± 2.46 |
Leaves | 14.80 b ± 0.85 | 16.90 b ± 2.91 | 18.50 b ± 1.49 | 76.80 a ± 1.59 | 78.40 a ± 4.18 | 81.40 a ± 4.28 | |
DHA (nmol g-1 FW) | Roots | 8.80 b ± 0.62 | 9.10 b ± 1.91 | 10.80 b ± 1.14 | 42.20 a ± 1.54 | 41.60 a ± 2.60 | 41.0 a ± 2.91 |
Leaves | 9.0 c ± 0.85 | 9.60 c ± 2.17 | 11.0 c ± 1.49 | 75.80 a ± 1.59 | 65.80 b ± 4.18 | 66.40 b ± 4.37 | |
AA/DHA | Roots | 1.15 b ± 0.01 | 1.24 a ± 0.04 | 1.18 b ± 0.02 | 1.06 d ± 0.01 | 1.11 c ± 0.01 | 1.17 b ± 0.03 |
Leaves | 1.64 b ± 0.06 | 1.77 a ± 0.10 | 1.69 ab ± 0.09 | 1.01 d ± 0.0 | 1.19 c ± 0.02 | 1.23 c ± 0.02 | |
GSH (nmol g−1 FW) | Roots | 25.0 bc ± 2.31 | 24.50 bc ± 2.44 | 23.0 c ± 2.95 | 28.90 a ± 0.82 | 27.20 ab ± 1.22 | 26.50 abc ± 0.89 |
Leaves | 32.90 bc ± 1.15 | 29.20 cd ± 1.78 | 26.80 d ± 1.56 | 38.50 a ± 2.29 | 33.90 b ± 2.71 | 30.90 bc ± 2.70 | |
GSSG (nmol g−1 FW) | Roots | 19.80 c ± 2.51 | 18.90 c ± 2.79 | 18.0 c ± 2.42 | 26.60 a ± 1.31 | 24.40 ab ± 1.56 | 21.80 bc ± 0.62 |
Leaves | 23.20 bc ± 1.15 | 18.10 d ± 2.01 | 18.0 d ± 1.40 | 34.40 a ± 2.80 | 25.30 b ± 2.55 | 19.70 cd ± 2.0 | |
GSH/GSSG | Roots | 1.27 ab ± 0.05 | 1.30 a ± 0.08 | 1.28 ab ± 0.01 | 1.09 c ± 0.03 | 1.11 c ± 0.02 | 1.21 b ± 0.04 |
Leaves | 1.42 cd ± 0.02 | 1.62 a ± 0.08 | 1.49 bc ± 0.05 | 1.12 e ± 0.04 | 1.34 d ± 0.03 | 1.57 ab ± 0.04 | |
Phenolics (mg g−1 DW) | Roots | 6.70 a ± 0.78 | 4.40 b ± 0.69 | 4.50 b ± 0.70 | 1.40 d ± 0.36 | 2.0 cd ± 0.69 | 2.80 c ± 0.60 |
Leaves | 13.0 a ± 1.40 | 10.40 b ± 0.85 | 9.20 b ± 0.87 | 3.30 d ± 0.36 | 4.0 d ± 0.72 | 7.10 c ± 1.13 |
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El-Dakak, R.; El-Aggan, W.; Badr, G.; Helaly, A.; Tammam, A. Positive Salt Tolerance Modulation via Vermicompost Regulation of SOS1 Gene Expression and Antioxidant Homeostasis in Viciafaba Plant. Plants 2021, 10, 2477. https://doi.org/10.3390/plants10112477
El-Dakak R, El-Aggan W, Badr G, Helaly A, Tammam A. Positive Salt Tolerance Modulation via Vermicompost Regulation of SOS1 Gene Expression and Antioxidant Homeostasis in Viciafaba Plant. Plants. 2021; 10(11):2477. https://doi.org/10.3390/plants10112477
Chicago/Turabian StyleEl-Dakak, Rehab, Weam El-Aggan, Ghadah Badr, Amira Helaly, and Amel Tammam. 2021. "Positive Salt Tolerance Modulation via Vermicompost Regulation of SOS1 Gene Expression and Antioxidant Homeostasis in Viciafaba Plant" Plants 10, no. 11: 2477. https://doi.org/10.3390/plants10112477