Aster glehni F. Schmidt Extract Modulates the Activities of HMG-CoA Reductase and Fatty Acid Synthase
Abstract
:1. Introduction
2. Results
2.1. HMG-CoA Reductase Inhibition Assay
2.2. Esterification of AGE
2.3. Concentration Dependent HMG-CoA Reductase Inhibition Assay
2.4. Concentration-Dependent Fatty Acid Synthase Inhibition Assay
2.5. Qualitative Analysis of AGEM and Quantitative Analysis of AGEM70M
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Instruments
4.3. Plant Materials
4.4. HMG-CoA Reductase (HMGR) Inhibition Assay
4.5. Esterification of the Ethyl Acetate Fractions of AG (AGE)
4.6. Fractionation of the Methylated AGE (AGEM)
4.7. Fatty Acid Synthase (FAS) Inhibition Assay
4.8. Qualitative Analysis of Components in AGEM
4.9. Quantitative Analysis of DCQA and Isoflavonoids in AGEM
Compound | m/z (Q1, Q3) | |
DCQA | 517.197 | 163.027 |
DCQA-ME | 531.177 | 163.000 |
Rhamnetin | 317.003 | 273.900 |
Isorhamnetin | 317.003 | 302.000 |
Kaempferol | 286.977 | 153.000 |
Quercetin | 302.950 | 229.000 |
Quercetin-d3 | 306.058 | 232.000 |
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Lee, H.; Kim, H.J.; Chae, H.; Yoon, N.E.; Jung, B.H. Aster glehni F. Schmidt Extract Modulates the Activities of HMG-CoA Reductase and Fatty Acid Synthase. Plants 2021, 10, 2287. https://doi.org/10.3390/plants10112287
Lee H, Kim HJ, Chae H, Yoon NE, Jung BH. Aster glehni F. Schmidt Extract Modulates the Activities of HMG-CoA Reductase and Fatty Acid Synthase. Plants. 2021; 10(11):2287. https://doi.org/10.3390/plants10112287
Chicago/Turabian StyleLee, Hyunbeom, Hyoung Ja Kim, Hyungi Chae, Na Eun Yoon, and Byung Hwa Jung. 2021. "Aster glehni F. Schmidt Extract Modulates the Activities of HMG-CoA Reductase and Fatty Acid Synthase" Plants 10, no. 11: 2287. https://doi.org/10.3390/plants10112287