Effects of Dipsacus asperoides and Phlomis umbrosa Extracts in a Rat Model of Osteoarthritis
Abstract
:1. Introduction
2. Results
2.1. Effects of D. asperoides Extract (DAE) and P. umbrosa Extract (PUE) on Body Weight and Serum Markers of Toxicity
2.2. Effects of DAE and PUE on Hind Paw Weight-Bearing Distribution in MIA-Induced OA Rats
2.3. Effects of DAE and PUE on Sezrum Levels of Cytokines in MIA-Induced OA Rats
2.4. Effects of DAE and PUE on the Histopathological Features of Joint Tissues in MIA-Induced OA Rats
2.5. Effects of DAE and PUE on MMP-2, MMP-9, and MMP-13 Expression in Knee Joint Tissues in MIA-Induced OA Rats
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Preparation of Herbal Extracts
4.3. Animals
4.4. Measurement of Hind Paw Weight-Bearing Distribution
4.5. Biochemical Blood Analysis
4.6. Histopathological Analysis
4.7. Immunohistochemical Analysis
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
ALT | Alanine aminotransferase |
AST | Aspartate aminotransferase |
DAE | Dipsacus asperoides extract |
H&E | Hematoxylin and eosin |
IL-1β | Interleukin-1 beta |
IM | Indomethacin |
KHP | Korean Herbal Pharmacopoeia |
MIA | Monosodium iodoacetate |
MMPs | Matrix metalloproteinases |
OA | Osteoarthritis |
PUE | Phlomis umbrosa extract |
Safranin O | Safranin O-fast green |
SCAR | Sequence-characterized amplified region |
TNF-α | Tumor necrosis factor alpha |
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Chun, J.M.; Lee, A.Y.; Moon, B.C.; Choi, G.; Kim, J.-S. Effects of Dipsacus asperoides and Phlomis umbrosa Extracts in a Rat Model of Osteoarthritis. Plants 2021, 10, 2030. https://doi.org/10.3390/plants10102030
Chun JM, Lee AY, Moon BC, Choi G, Kim J-S. Effects of Dipsacus asperoides and Phlomis umbrosa Extracts in a Rat Model of Osteoarthritis. Plants. 2021; 10(10):2030. https://doi.org/10.3390/plants10102030
Chicago/Turabian StyleChun, Jin Mi, A Yeong Lee, Byeong Cheol Moon, Goya Choi, and Joong-Sun Kim. 2021. "Effects of Dipsacus asperoides and Phlomis umbrosa Extracts in a Rat Model of Osteoarthritis" Plants 10, no. 10: 2030. https://doi.org/10.3390/plants10102030