Molecular Phylogeny of Trifolium L. Section Trifolium with Reference to Chromosome Number and Subsections Delimitation
Abstract
:1. Introduction
2. Results
3. Discussion
4. Material and Methods
4.1. Plant Material
4.2. DNA Isolation and DNA Sequence
4.3. Phylogenetic Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Trifolium Species | 2n Chromosome Number | Subsection | New Clade |
---|---|---|---|
T. alexandrinum | 16 | Alexandrina | Clade I |
T. vavilovii | 16 | ||
T. apertum | 16 | ||
T. berytheum | 16 | ||
T. meironense | 16 | ||
T. scutatum | 10, 16 | Clypeata | |
T. clypeatum | 16 | ||
T. plebeium | 16 | ||
T. pallidum | 16 | Trifolium | |
T. lucanicum | - | Scabroidea | |
T. dasyurum | 16 | Angustifolia | |
T. dichroanthum | 16 | ||
T. palaestinum | 16 | ||
T. angustifolium | 14, 16, 32 | ||
T. purpureum | 14, 16 | ||
T. carmeli | 14 | Echinata | |
T. echinatum | 16 | ||
T. canescens | - | Ochroleuca | |
T. caucasicum | - | ||
T. pannonicum | 64, 96, 98~180 | ||
T. miegeanum | 16 | Urceolata | |
T. obscurum | 16 | ||
T. squarrosum | 16 | ||
T. constantinopolitanum | 16 | ||
T. striatum | 14 | Stenosemium | Clad II |
T. latinum | 16 | Echinata | |
T. alpestre | 16 | Alpesteria | |
T. rubens | 16 | ||
T. medium | 48, 64, 78, 80, 84, 96, 98, 126 | Intermedia | |
T. heldreichianum | 16 | ||
T. patulum | - | ||
T. velebiticum | - | ||
T. wettsteinii | - | ||
T. caudatum | - | Ochroleuca | |
T. longidentatum | - | ||
T. trichocephalum | 14, 28 | ||
T. ochroleucum | 16 | ||
T. gemellum | 14 | Pheloidea | |
T. phleoides | 14 | ||
T. ligusticum | 12 | ||
T. leucanthum | 14, 16 | Urceolata | |
T. diffusum | 16 | Trifolium | |
T. pratense | 14, 16, 28, 56 | ||
T. molinerii | 14 | Stellata | |
T. incarnatum | 14 | ||
T. stellatum | 14 | ||
T. lappaceum | 16 | Lappacea | |
T. affine | 12, 14, 16 | Arvensia | |
T. arvense | 14 | ||
T. bocconei | 12, 14 | Trichoptera | |
T. dalmaticum | 10 | Scabroidea | |
T. sylvaticum | 14, 16 | Stellata | Clade III |
T. hirtum | 10 | Lappacea | |
T. cherleri | 10 | ||
T. haussknechtii | 16 | Angustifolia | |
T. scabrum | 10, 16 | Scabroidea | |
T. trichopterum | 14 | Trichoptera |
No | Trifolium Species | Source and Accession Number * | Country of Origin | Gene Bank ITS1/ITS2 |
---|---|---|---|---|
1 | T. affine Presl. | SRPIS 369014 | Turkey | MW683982/MW684022 |
2 | T. alexandrinum L. | ARC, EG 10 | Egypt | AF154381/AF154605 |
3 | T. alpestre L. | Kew 11097 | Switzerland | AF154400/AF154624 |
4 | T. angustifolium L. | MU 9491 | USA | MW683983/MW684023 |
5 | T. angustifolium L. | BGUZ 8023 | Switzerland | AF154388/AF154612 |
6 | T. apertum Bobrov | SRPIS 314117 | Russia | MW683984/MW684024 |
7 | T. arvense L. | SRPIS 120077 | Turkey | MW683985/MW684025 |
8 | T. berytheum Bois. and T. Blanche | SRPIS 369019 | Turkey | MW683986/MW684026 |
9 | T. bocconei Savi | SRPIS 224643 | Morocco | MW683987/MW684027 |
10 | T. canescens Willd. | WRPIS 418866 | Italy | MW683988/MW684028 |
11 | T. carmeli Boiss. | IPK TRIF 100/75 | USA | MW683989/MW684029 |
12 | T. carmeli | SRPIS 353422 | Israel | MW683990/MW684030 |
13 | T. caucasicum Tausch* | WRPIS 597496 | Russia | MW683991/MW684031 |
14 | T. caudatum Boiss. | WRPIS 251858 | Italy | MW683992/MW684032 |
15 | T. cherleri L. | Italy 589 | Italy | AF154357/AF154582 |
16 | T. clypeatum L. | ICARDA 1149 | Syria | AF154383/AF154607 |
17 | T. constantinopolitanum Sert. | ICARDA 1114 | Syria | AF154386/AF154610 |
18 | T. dalmaticum Vis. | WRPIS 516292 | Serbia | MW683993/MW684033 |
19 | T. dasyurum C. Presl. | SRPIS 369029 | Australia | AF154387/AF154611 |
20 | T. dichroanthum Boiss. | SRPIS 292474 | Israel | MW683994/MW684034 |
21 | T. diffusum Ehrh. | Kew 1094 | Serbia | AF154401/AF154625 |
22 | T. echinatum N.B. | ICARDA 1758 | Syria | AF154382/AF154606 |
23 | T. gemellum Willd. | SRPIS 302967 | Spain | MW683995/MW684035 |
24 | T. haussknechtii Boiss. | WRPIS G31176-98i | Bulgaria | MW683996/MW684036 |
25 | T. heldreichianum Hausskn. | WRPIS 419289 | Greece | MW683997/MW684037 |
26 | T. hirtum All. | Kew 25085 | Greece | AF154359/AF154932 |
27 | T. incarnatum L. | Kew 55767 | England | AF154392/AF154932 |
28 | T. lappaceum L. | ICARDA 108405 | Syria | MW683998/MW684038 |
29 | T. lappaceum L.. | Italy 529 | Italy | AF154395/AF154619 |
30 | T. latinum Sebast. | IOWA State | USA | MW683999/MW684039 |
31 | T. leucanthum M.B. | ICARDA 1111 | Syria | AF154394/AF154618 |
32 | T. ligusticum Lois. | SRPIS 419415 | Greece | MW684000/MW684040 |
33 | T. longidentatum Nabelek | WRPIS 542831 | Bosnia, Herzegovina | MW684001/MW684041 |
34 | T. lucanicum Gasp. Ex Guss. | Kew 13921 | Greece | AF154390/AF154614 |
35 | T. medium. L. | WRPIS 325481 | Russia | MW684002/MW684042 |
36 | T. meironense Zoh. and Lern. | ICARDA 69062 | Algeria | MW684003/MW684043 |
37 | T. miegeanum Maire | SRPIS 258406 | Portugal | MW684004/MW684044 |
38 | T. molinerii (incarnatum L.) | SRPIS 591667 | Bulgaria | MW684005/MW684045 |
39 | T. obscurum Savi | SRPIS 369057 | Italy | MW684006/MW684046 |
40 | T. ochroleucum Huds. | Kew 7559 | Greece | AF154397/AF154621 |
41 | T. palaestinum Boiss. | SRPIS 369060 | Palestine | MW684007/MW684047 |
42 | T. pallidum Waldst. and Kit. | Kew 31516 | Greece | AF154385/AF154609 |
43 | T. pannonicum Jacq. | BGUZ s.n. | Switzerland | AF154384/AF154608 |
44 | T. patulum Tausch | WRPIS 604678-97i | Serbia | MW684008/MW684048 |
45 | T. phleoides Willd. | SRPIS 298420 | Turkey | MW684009/MW684049 |
46 | T. plebeium Boiss. | ICARDA 67905 | Syria | MW684010/MW684050 |
47 | T. pratense L. | JBVU 1044 | France | AF154396/AF154620 |
48 | T. purpureum Loisel | ICARDA 938 | Syria | AF154389/AF154613 |
49 | T. rubens L. | IPP 135 | France | AF154398/AF154622 |
50 | T. scabrum L. | ICARDA 939 | Syria | AF154358/AF154583 |
51 | T. scutatum Boiss. | ICARDA 67459 | Syria | MW684011/MW684051 |
52 | T. squarrosum L. | SRPIS 233720 | Italy | MW684012/MW684052 |
53 | T. stellatum L. | ICARDA 947 | Syria | AF154393/AF154617 |
54 | T. striatum L. | Kew 5337 | England | AF154391/AF154615 |
55 | T. sylvaticum Gerard | SRPIS 120194 | Turkey | MW684013/MW684053 |
56 | T. trichocephalum Bieb. | WRPIS 251210-64 | North Macedonia | MW684014/MW684054 |
57 | T. trichopterum Pancic | SRPIS 583430 | Bulgaria | MW684015/MW684055 |
58 | T. vavilovii Eig | Vencent 11160 | USA | MW684016/MW684056/ |
59 | T. velebiticum Degen | WRPIS G30679-97i | USA | MW684017/MW684057 |
60 | T. wettsteinii Dorfl. and Hay | WRPIS G31121 | USA | MW684018/MW684058 |
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Ahmed, H.I.S.; Badr, A.; El-Shazly, H.H.; Watson, L.; Fouad, A.S.; Ellmouni, F.Y. Molecular Phylogeny of Trifolium L. Section Trifolium with Reference to Chromosome Number and Subsections Delimitation. Plants 2021, 10, 1985. https://doi.org/10.3390/plants10101985
Ahmed HIS, Badr A, El-Shazly HH, Watson L, Fouad AS, Ellmouni FY. Molecular Phylogeny of Trifolium L. Section Trifolium with Reference to Chromosome Number and Subsections Delimitation. Plants. 2021; 10(10):1985. https://doi.org/10.3390/plants10101985
Chicago/Turabian StyleAhmed, Hanan I. Sayed, Abdelfattah Badr, Hanaa H. El-Shazly, Linda Watson, Ahmed S. Fouad, and Faten Y. Ellmouni. 2021. "Molecular Phylogeny of Trifolium L. Section Trifolium with Reference to Chromosome Number and Subsections Delimitation" Plants 10, no. 10: 1985. https://doi.org/10.3390/plants10101985