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Open AccessArticle

Predominance and Diversity of GLRaV-3 in Native Vines of Mediterranean Croatia

1
Institute for Adriatic Crops, 21000 Split, Croatia
2
CNR, Institute for Sustainable Plant Protection, 70126 Bari, Italy
3
Department of Biology, Faculty of Science, University of Zagreb, 10000 Zagreb, Croatia
*
Author to whom correspondence should be addressed.
Plants 2021, 10(1), 17; https://doi.org/10.3390/plants10010017
Received: 1 December 2020 / Revised: 15 December 2020 / Accepted: 21 December 2020 / Published: 24 December 2020
(This article belongs to the Special Issue Grapevine–Cooperation and Struggle with Microbial Residents)
Sixteen grapevine cultivars from Mediterranean Croatia were surveyed for the presence of 10 of the most economically important grapevine viruses. The presence of Grapevine fanleaf virus (GFLV), Arabis mosaic virus (ArMV), Grapevine leafroll associated virus-1, -2, and -3 (GLRaV-1; GLRaV-2 and GLRaV-3), Grapevine virus A (GVA) and B (GVB), Grapevine fleck virus (GFkV), Grapevine rupestris stem pitting associated virus (GRSPaV), and Grapevine Pinot gris virus (GPGV) were tested by reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). All 71 analyzed clones were positive for the presence of one or more viruses. The most abundant one, detected in almost 95% of samples was GLRaV-3. In most of cases it was reported in mixed infections with GVA, GRSPaV, and GPGV. Virus genomes of GLRaV-3 infected vines were further characterized molecularly in order to determine their genetic diversity. Different genomic variants of heat shock 70 protein homologue (HSP70h) were identified by single-strand conformation polymorphism (SSCP) and sequenced. Sequence analysis confirmed their clustering into phylogenetic group I and/or phylogenetic group II. This study emphasizes the wide virus heterogenicity in Mediterranean vines and the predominant presence of GLRaV-3 phylogenetic groups I and II, either individually or in combination. View Full-Text
Keywords: grapevine viruses; leafroll; autochthonous cultivars; screening; molecular variants grapevine viruses; leafroll; autochthonous cultivars; screening; molecular variants
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MDPI and ACS Style

Hančević, K.; Saldarelli, P.; Čarija, M.; Černi, S.; Zdunić, G.; Mucalo, A.; Radić, T. Predominance and Diversity of GLRaV-3 in Native Vines of Mediterranean Croatia. Plants 2021, 10, 17. https://doi.org/10.3390/plants10010017

AMA Style

Hančević K, Saldarelli P, Čarija M, Černi S, Zdunić G, Mucalo A, Radić T. Predominance and Diversity of GLRaV-3 in Native Vines of Mediterranean Croatia. Plants. 2021; 10(1):17. https://doi.org/10.3390/plants10010017

Chicago/Turabian Style

Hančević, Katarina; Saldarelli, Pasquale; Čarija, Mate; Černi, Silvija; Zdunić, Goran; Mucalo, Ana; Radić, Tomislav. 2021. "Predominance and Diversity of GLRaV-3 in Native Vines of Mediterranean Croatia" Plants 10, no. 1: 17. https://doi.org/10.3390/plants10010017

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