Predominance and Diversity of GLRaV-3 in Native Vines of Mediterranean Croatia
Abstract
:1. Introduction
2. Results
2.1. RT-PCR and ELISA Virus Detection
2.2. SSCP Analysis and Molecular Diversity of GLRaV-3
3. Discussion
3.1. Virus Distribution
3.2. Study of GLRaV-3 Natural Population
4. Materials and Methods
4.1. Viral Source
4.2. ELISA Virus Detection
4.3. RNA Extractions, cDNA Synthesis and PCR Virus Detection
4.4. Cloning and Single Stranded Conformational Polymorphism Analysis
4.5. Nucleotide Sequence Analysis
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Cultivar | Vine-Growing Area | Isolate Name | GLRaV-3 Phylogenetic Group | Co-Occurrence of Other Viruses | Leafroll Symptoms Presence | |
---|---|---|---|---|---|---|
Babica crna | Kaštela | BC1 | I | GVA,GVB,GFkV,GRSPaV | − | |
Babica crna | Kaštela | BC3 | I | GVA,GVB,GFkV,GPGV,GRSPaV | − | |
Babić | Primošten | Bab5 | I+II | GLRaV-2,GVA,GFkV,GPGV,GRSPaV | + | |
Babić | Primošten | Bab6 | I+II | GLRaV-2,GVA,GFkV,GPGV,GRSPaV | + | |
Crljenak kaštelanski | Kaštela | CK_092/4 | CK_093/2 | I+II | GVA,GFLV,ArMV,GFkV,GRSPaV | + |
Crljenak kaštelanski | Kaštela | I | GVA,GPGV,GRSPaV | + | ||
Dobričić | Šolta | Dob6 | II | GLRaV-1,GVA,GRSPaV | + | |
Dobričić | Šolta | Dob7 | II | GLRaV-1,GVA,GRSPaV | + | |
Glavinuša | Kaštela | Gla1 | II | GVA,GPGV,GRSPaV | + | |
Glavinuša | Kaštela | Gla5 | II | GVA,GPGV,GRSPaV | + | |
Grk | Korčula | Grk1 | II | GRSPaV | + | |
Grk | Korčula | Grk4 | II | GPGV,GRSPaV | + | |
Kujunduša | Imotski | K3 | II | GLRaV-2,GVA,GRSPaV | + | |
Kujunđuša | Imotski | K4 | II | GVA,GRSPaV | + | |
Malvasia dubr. | Konavle | Mal2 | I+II | GLRaV-1,GVA,GVB,GPGV,GRSPaV | + | |
Malvasia dubr. | Konavle | Mal4 | I | GLRaV-1,GVA,GVB,GRSPaV | + | |
Malvasia dubr. | Konavle | Mal6 | I+II | GLRaV-1,GVA,GVB,GPGV,GRSPaV | + | |
Plavac mali | Vis | 217/1 | II | − | ||
Plavac mali | Vis | 217/2 | II | − | ||
Plavac mali | Vis | 217/3 | II | GPGV | − | |
Plavina | Drniš | Pla1 | I | GVA,GPGV,GRSPaV | + | |
Pošip bijeli | Korčula | PB1 | I | GLRaV-1,GVA,GPGV,GRSPaV | − | |
Pošip bijeli | Korčula | PB5 | I | GLRaV-1,GVA,GPGV,GRSPaV | − | |
Pošip crni | Korčula | PC4 | I | GLRaV-1,GPGV,GRSPaV | − | |
Prč | Hvar | Prc5 | I | GLRaV-1,GVA | − | |
Prč | Hvar | Prc6 | II | GVA,GRSPaV | − | |
Rukatac | Korčula | Ruk4 | II | GVA | + | |
Rukatac | Korčula | Ruk6 | II | GLRaV-1,GVA,GRSPaV | + | |
Vugava | Vis | Vu5 | II | GLRaV-1,GVA,GFLV | + | |
Vugava | Vis | Vu6 | II | GLRaV-1,GVA,GFLV,GRSPaV | + | |
Vugava | Vis | Vu7 | I+II | GLRaV1,GVA,GFLV,GFkV,GPGV,GRSPaV | + | |
Zlatarica vrgorska | Vrgorac | ZV2 | II | GVA,GPGV,GRSPaV | − | |
Zlatarica vrgorska | Vrgorac | ZV3 | II | GVA,GRSPaV | − |
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Hančević, K.; Saldarelli, P.; Čarija, M.; Černi, S.; Zdunić, G.; Mucalo, A.; Radić, T. Predominance and Diversity of GLRaV-3 in Native Vines of Mediterranean Croatia. Plants 2021, 10, 17. https://doi.org/10.3390/plants10010017
Hančević K, Saldarelli P, Čarija M, Černi S, Zdunić G, Mucalo A, Radić T. Predominance and Diversity of GLRaV-3 in Native Vines of Mediterranean Croatia. Plants. 2021; 10(1):17. https://doi.org/10.3390/plants10010017
Chicago/Turabian StyleHančević, Katarina, Pasquale Saldarelli, Mate Čarija, Silvija Černi, Goran Zdunić, Ana Mucalo, and Tomislav Radić. 2021. "Predominance and Diversity of GLRaV-3 in Native Vines of Mediterranean Croatia" Plants 10, no. 1: 17. https://doi.org/10.3390/plants10010017
APA StyleHančević, K., Saldarelli, P., Čarija, M., Černi, S., Zdunić, G., Mucalo, A., & Radić, T. (2021). Predominance and Diversity of GLRaV-3 in Native Vines of Mediterranean Croatia. Plants, 10(1), 17. https://doi.org/10.3390/plants10010017