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Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish

Department of Biomedical Science, The University of Sheffield, Western Bank, Sheffield S10 2TN, UK
Department of Infection, Immunity and Cardiovascular Disease, The University of Sheffield, Western Bank Sheffield, S10 2TN, UK
Authors to whom correspondence should be addressed.
J. Dev. Biol. 2018, 6(1), 6;
Received: 6 February 2018 / Revised: 1 March 2018 / Accepted: 6 March 2018 / Published: 9 March 2018
(This article belongs to the Special Issue Zebrafish - A Model System for Developmental Biology Study)
The zebrafish is an established model to study the development and function of visual neuronal circuits in vivo, largely due to their optical accessibility at embryonic and larval stages. In the past decade multiple experimental paradigms have been developed to study visually-driven behaviours, particularly those regulated by the optic tectum, the main visual centre in lower vertebrates. With few exceptions these techniques are limited to young larvae (7–9 days post-fertilisation, dpf). However, many forms of visually-driven behaviour, such as shoaling, emerge at later developmental stages. Consequently, there is a need for an experimental paradigm to image the visual system in zebrafish larvae beyond 9 dpf. Here, we show that using NBT:GCaMP3 line allows for imaging neuronal activity in the optic tectum in late stage larvae until at least 21 dpf. Utilising this line, we have characterised the receptive field properties of tectal neurons of the 2–3 weeks old fish in the cell bodies and the neuropil. The NBT:GCaMP3 line provides a complementary approach and additional opportunities to study neuronal activity in late stage zebrafish larvae. View Full-Text
Keywords: zebrafish; calcium imaging; neuronal activity zebrafish; calcium imaging; neuronal activity
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MDPI and ACS Style

Bergmann, K.; Meza Santoscoy, P.; Lygdas, K.; Nikolaeva, Y.; MacDonald, R.B.; Cunliffe, V.T.; Nikolaev, A. Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish. J. Dev. Biol. 2018, 6, 6.

AMA Style

Bergmann K, Meza Santoscoy P, Lygdas K, Nikolaeva Y, MacDonald RB, Cunliffe VT, Nikolaev A. Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish. Journal of Developmental Biology. 2018; 6(1):6.

Chicago/Turabian Style

Bergmann, Katharina; Meza Santoscoy, Paola; Lygdas, Konstantinos; Nikolaeva, Yulia; MacDonald, Ryan B.; Cunliffe, Vincent T.; Nikolaev, Anton. 2018. "Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish" J. Dev. Biol. 6, no. 1: 6.

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