Appropriate Amounts and Activity of the Wilms’ Tumor Suppressor Gene, wt1, Are Required for Normal Pronephros Development of Xenopus Embryos
Abstract
:1. Introduction
2. Materials and Methods
2.1. Embryos and Microinjection
2.2. Whole-Mount In Situ Hybridization (WISH)
2.3. Beta-Gal Staining
2.4. CRISPR/Cas9 Knockout of wt1 and tyrosinase
2.5. Plasmid Construction and mRNA Synthesis
2.6. Luciferase Reporter Assay
3. Results
3.1. Reduced Expression of the Pronephros Marker Genes in the wt1 Knockout Embryos by CRISPR/Cas9 Method
3.2. Transcriptional Activity of the WT1 in Xenopus Embryo
3.3. Pronephric Phenotypes of the wt1 mRNA Injected Embryos
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Shiraki, T.; Hayashi, T.; Ozue, J.; Watanabe, M. Appropriate Amounts and Activity of the Wilms’ Tumor Suppressor Gene, wt1, Are Required for Normal Pronephros Development of Xenopus Embryos. J. Dev. Biol. 2022, 10, 46. https://doi.org/10.3390/jdb10040046
Shiraki T, Hayashi T, Ozue J, Watanabe M. Appropriate Amounts and Activity of the Wilms’ Tumor Suppressor Gene, wt1, Are Required for Normal Pronephros Development of Xenopus Embryos. Journal of Developmental Biology. 2022; 10(4):46. https://doi.org/10.3390/jdb10040046
Chicago/Turabian StyleShiraki, Taisei, Takuma Hayashi, Jotaro Ozue, and Minoru Watanabe. 2022. "Appropriate Amounts and Activity of the Wilms’ Tumor Suppressor Gene, wt1, Are Required for Normal Pronephros Development of Xenopus Embryos" Journal of Developmental Biology 10, no. 4: 46. https://doi.org/10.3390/jdb10040046