Involvement of a Basic Helix-Loop-Helix Gene BHLHE40 in Specification of Chicken Retinal Pigment Epithelium
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chicken Embryos
2.2. Isolation of Chicken cDNAs and Construction of Expression Vectors
2.3. Cryosectioning
2.4. In Situ Hybridization (ISH)
2.5. Western Blot Analysis
2.6. In Ovo Electroporation
2.7. Immunofluorescence
2.8. Image Capture and Processing
3. Results
3.1. Expression Patterns of BHLHE40 in Chickens’ Early Eye Development
3.2. Overexpression of BHLHE40 in the OV Drives Early RPE Development
3.3. Effects of LHX1 Overexpression on BHLHE40 Expression
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kinuhata, T.; Sato, K.; Bando, T.; Mito, T.; Miyaishi, S.; Nohno, T.; Ohuchi, H. Involvement of a Basic Helix-Loop-Helix Gene BHLHE40 in Specification of Chicken Retinal Pigment Epithelium. J. Dev. Biol. 2022, 10, 45. https://doi.org/10.3390/jdb10040045
Kinuhata T, Sato K, Bando T, Mito T, Miyaishi S, Nohno T, Ohuchi H. Involvement of a Basic Helix-Loop-Helix Gene BHLHE40 in Specification of Chicken Retinal Pigment Epithelium. Journal of Developmental Biology. 2022; 10(4):45. https://doi.org/10.3390/jdb10040045
Chicago/Turabian StyleKinuhata, Toshiki, Keita Sato, Tetsuya Bando, Taro Mito, Satoru Miyaishi, Tsutomu Nohno, and Hideyo Ohuchi. 2022. "Involvement of a Basic Helix-Loop-Helix Gene BHLHE40 in Specification of Chicken Retinal Pigment Epithelium" Journal of Developmental Biology 10, no. 4: 45. https://doi.org/10.3390/jdb10040045