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Beyond the Scavenging of Reactive Oxygen Species (ROS): Direct Effect of Cerium Oxide Nanoparticles in Reducing Fatty Acids Content in an In Vitro Model of Hepatocellular Steatosis

1
Service of Biochemistry and Molecular Genetics, Hospital Clinic Universitari, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), 08905 Barcelona, Spain
2
School of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529020, China
3
Departament of Biomedicine, University of Barcelona, 08905 Barcelona, Spain
4
Working group for the biochemical assessment of hepatic disease-SEQCML, 08036 Barcelona, Spain
5
Vall d’Hebron Research Institute (VHIR), 08035 Barcelona, Spain
6
Institut Català de Nanociència i Nanotecnologia (ICN2), CSIC, The Barcelona Institute of Science and Technology (BIST), Campus UAB, Bellaterra, 08193 Barcelona, Spain
7
Institució Catalana de Recerca i Estudis Avançats (ICREA), 08010 Barcelona, Spain
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Biomolecules 2019, 9(9), 425; https://doi.org/10.3390/biom9090425
Received: 13 July 2019 / Revised: 9 August 2019 / Accepted: 27 August 2019 / Published: 29 August 2019
Nonalcoholic fatty liver disease (NAFLD) is characterized by hepatic accumulation of lipids. Antisteatotic effects of cerium oxide nanoparticles (CeO2NPs) have recently been shown in animal models of liver disease. However, it is unclear whether the activity of CeO2NPs is related solely to the decrease in oxidative stress or, in addition, they directly decrease liver fatty acid accumulation. To address this question, in this work, we used an in vitro model of hepatocellular steatosis, exposing HepG2 cells to oleic and palmitic acid. Cell uptake of CeO2NPs and their effect on oxidative stress and viability of hepatic cells cultured with H2O2 were also evaluated. Results show that CeO2NPs were uptaken by HepG2 cells and reduced oxidative stress and improved cell viability. Treatment with oleic and palmitic acid increased lipogenesis and the content of different fatty acids. CeO2NPs reduced palmitic and stearic acid and most fatty acids consisting of more than 18 carbon atoms. These effects were associated with significant changes in elongase and desaturase activity. In conclusion, CeO2NPs directly protected HepG2 cells from cell injury in oxidative stress conditions and reduced fatty acid content in steatotic conditions by inducing specific changes in fatty acid metabolism, thus showing potential in the treatment of NAFLD. View Full-Text
Keywords: nonalcoholic fatty liver disease; steatosis; liver; cerium oxide nanoparticles; oxidative stress nonalcoholic fatty liver disease; steatosis; liver; cerium oxide nanoparticles; oxidative stress
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MDPI and ACS Style

Parra-Robert, M.; Casals, E.; Massana, N.; Zeng, M.; Perramón, M.; Fernández-Varo, G.; Morales-Ruiz, M.; Puntes, V.; Jiménez, W.; Casals, G. Beyond the Scavenging of Reactive Oxygen Species (ROS): Direct Effect of Cerium Oxide Nanoparticles in Reducing Fatty Acids Content in an In Vitro Model of Hepatocellular Steatosis. Biomolecules 2019, 9, 425. https://doi.org/10.3390/biom9090425

AMA Style

Parra-Robert M, Casals E, Massana N, Zeng M, Perramón M, Fernández-Varo G, Morales-Ruiz M, Puntes V, Jiménez W, Casals G. Beyond the Scavenging of Reactive Oxygen Species (ROS): Direct Effect of Cerium Oxide Nanoparticles in Reducing Fatty Acids Content in an In Vitro Model of Hepatocellular Steatosis. Biomolecules. 2019; 9(9):425. https://doi.org/10.3390/biom9090425

Chicago/Turabian Style

Parra-Robert, Marina; Casals, Eudald; Massana, Nuria; Zeng, Muling; Perramón, Meritxell; Fernández-Varo, Guillermo; Morales-Ruiz, Manuel; Puntes, Víctor; Jiménez, Wladimiro; Casals, Gregori. 2019. "Beyond the Scavenging of Reactive Oxygen Species (ROS): Direct Effect of Cerium Oxide Nanoparticles in Reducing Fatty Acids Content in an In Vitro Model of Hepatocellular Steatosis" Biomolecules 9, no. 9: 425. https://doi.org/10.3390/biom9090425

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