The Effect of Buffers on Weak Acid Uptake by Vesicles
AbstractThe assessment of weak acid membrane permeability (Pm) frequently involves large unilamellar vesicles. It relies on measurements of the intravesicular pH drop, ΔpHin, in response to a sudden augmentation of external acid concentration. However, ΔpHin may be primarily governed by non-instantaneous protonation and deprotonation reactions of (i) the acid itself, (ii) the buffer molecules, and (iii) the fluorescent pH reporter dye. Moreover, buffer concentration and acid gradient also serve as determinants of ΔpHin, as we show here. The uniexponential time constant (τ) of ΔpHin(t) is an invalid measure of Pm as Arrhenius plots of Pm and
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Hannesschlaeger, C.; Barta, T.; Pechova, H.; Pohl, P. The Effect of Buffers on Weak Acid Uptake by Vesicles. Biomolecules 2019, 9, 63.
Hannesschlaeger C, Barta T, Pechova H, Pohl P. The Effect of Buffers on Weak Acid Uptake by Vesicles. Biomolecules. 2019; 9(2):63.Chicago/Turabian Style
Hannesschlaeger, Christof; Barta, Thomas; Pechova, Hana; Pohl, Peter. 2019. "The Effect of Buffers on Weak Acid Uptake by Vesicles." Biomolecules 9, no. 2: 63.
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