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Biomolecules 2019, 9(2), 50; https://doi.org/10.3390/biom9020050

Heat Shock Protein 27 Phosphorylation Regulates Tumor Cell Migration under Shear Stress

1
School of Biomedical Engineering, Dalian University of Technology, Liaoning IC Technology Key Lab, Dalian 116024, China
2
Institute of Biomedical Engineering, Chongqing University of Science and Technology, Chongqing 401331, China
*
Author to whom correspondence should be addressed.
Received: 25 October 2018 / Revised: 25 January 2019 / Accepted: 25 January 2019 / Published: 30 January 2019
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Abstract

Heat shock protein 27 (HSP27) is a multifunctional protein that undergoes significant changes in its expression and phosphorylation in response to shear stress stimuli, suggesting that it may be involved in mechanotransduction. However, the mechanism of HSP27 affecting tumor cell migration under shear stress is still not clear. In this study, HSP27-enhanced cyan fluorescent protein (ECFP) and HSP27-Ypet plasmids are constructed to visualize the self-polymerization of HSP27 in living cells based on fluorescence resonance energy transfer technology. The results show that shear stress induces polar distribution of HSP27 to regulate the dynamic structure at the cell leading edge. Shear stress also promotes HSP27 depolymerization to small molecules and then regulates polar actin accumulation and focal adhesion kinase (FAK) polar activation, which further promotes tumor cell migration. This study suggests that HSP27 plays an important role in the regulation of shear stress-induced HeLa cell migration, and it also provides a theoretical basis for HSP27 as a potential drug target for metastasis. View Full-Text
Keywords: heat shock protein 27; shear stress; migration; phosphorylation; signaling pathway heat shock protein 27; shear stress; migration; phosphorylation; signaling pathway
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Zhang, B.; Xie, F.; Aziz, A.R.; Shao, S.; Li, W.; Deng, S.; Liao, X.; Liu, B. Heat Shock Protein 27 Phosphorylation Regulates Tumor Cell Migration under Shear Stress. Biomolecules 2019, 9, 50.

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