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Open AccessArticle

Mechanism of Action and Interactions between Thyroid Peroxidase and Lipoxygenase Inhibitors Derived from Plant Sources

1
Department of Biochemistry and Food Chemistry, University of Life Sciences, Skromna Str. 8, 20-704 Lublin, Poland
2
Department of Thermal Technology and Food Process Engineering, University of Life Sciences, Głęboka 31, 20-612 Lublin, Poland
*
Author to whom correspondence should be addressed.
Biomolecules 2019, 9(11), 663; https://doi.org/10.3390/biom9110663
Received: 28 July 2019 / Revised: 1 October 2019 / Accepted: 17 October 2019 / Published: 29 October 2019
This study focused on the effect of kaempferol, catechin, apigenin, sinapinic acid, and extracts from plants (i.e., parsley, cumin, mustard, green tea, and green coffee) on thyroid peroxidase (TPO) and lipoxygenase (LOX) activity, antiradical potential, as well as the result of interactions among them. Catechin, sinapinic acid, and kaempferol acted as a competitive TPO inhibitors, while apigenin demonstrated an uncompetitive mode of inhibitory action. Ethanol extracts from all plants acted as competitive TPO inhibitors, while, after in vitro digestion, TPO activation was found especially in the case of mustard (24%) and cumin (19.85%). Most importantly, TPO activators acted synergistically. The TPO effectors acted as LOX inhibitors. The most effective were potentially bioaccessible compounds from green tea and green coffee (IC50 = 29.73 mg DW/mL and 30.43 mg DW/mL, respectively). The highest free radical scavenging ability was determined for catechin and sinapinic acid (IC50 = 78.37 µg/mL and 84.33 µg/mL, respectively) and potentially bioaccessible compounds from mustard (0.42 mg DW/mL) and green coffee (0.87 mg DW/mL). Green coffee, green tea, cumin, and mustard contain potentially bioaccessible TPO activators that also act as effective LOX inhibitors, which indicate their potentially health-promoting effects for people suffering from Hashimoto’s disease. View Full-Text
Keywords: thyroid peroxidase; lipoxygenase; inhibition; dietary polyphenols; antioxidant activity thyroid peroxidase; lipoxygenase; inhibition; dietary polyphenols; antioxidant activity
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MDPI and ACS Style

Habza-Kowalska, E.; Gawlik-Dziki, U.; Dziki, D. Mechanism of Action and Interactions between Thyroid Peroxidase and Lipoxygenase Inhibitors Derived from Plant Sources. Biomolecules 2019, 9, 663.

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