Cell-Type-Specific Neuroproteomics of Synapses
Abstract
:1. Introduction
2. Synapses
2.1. Structure of Synapses
2.2. Isolation of Synapses
3. Advancements in Neuroproteomics
3.1. Isolation of Cell Types, Subcellular Compartments, and Cell-Type-Specific Synapses
3.1.1. Transgenic Animals
3.1.2. Laser Capture Microdissection (LCM)
3.1.3. Magnetic-Activated Cell Sorting (MACS)
3.1.4. Fluorescence-Activated Cell Sorting (FACS)
3.1.5. Tandem Affinity Purification
3.1.6. Protein Labeling
3.2. Advancements in MS Approaches
3.2.1. Direct In Situ Spatial Proteomics
3.2.2. Single-Cell Mass Spectrometry
4. Application of Neuroproteomic Analysis to Neuropsychiatric Disorders
4.1. Autism Spectrum Disorder
4.2. Alzheimer’s Disease
4.3. Schizophrenia
4.4. Major Depressive Disorder
4.5. Substance Use Disorders
5. Limitations and Future Perspectives
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Yim, Y.Y.; Nestler, E.J. Cell-Type-Specific Neuroproteomics of Synapses. Biomolecules 2023, 13, 998. https://doi.org/10.3390/biom13060998
Yim YY, Nestler EJ. Cell-Type-Specific Neuroproteomics of Synapses. Biomolecules. 2023; 13(6):998. https://doi.org/10.3390/biom13060998
Chicago/Turabian StyleYim, Yun Young, and Eric J. Nestler. 2023. "Cell-Type-Specific Neuroproteomics of Synapses" Biomolecules 13, no. 6: 998. https://doi.org/10.3390/biom13060998