Molecular Recognition of Proteins through Quantitative Force Maps at Single Molecule Level
Abstract
:1. Introduction
2. Materials and Methods
2.1. Protein Labeling and Covalent Immobilization on Mica
2.2. HRP–Biotin Enzymatic Assays
2.3. Topography and Adhesion AFM Mapping Analysis
3. Results
3.1. HRP–Biotin Enzymatic Assays
3.2. AFM Measurements
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Control with Mica without Protein | Control of HRP–Biotin without TMB | |||
---|---|---|---|---|
Without Protein | Avidin 2 μg | Streptavidin 2 μg | ||
Absorbance 450 nm | 0.6367 | 0.0030 | 0.0024 | 0.0032 |
Incubated Protein (µg) | Avidin Absorbance (450 nm) | Avidin Molecules/cm2 | Streptavidin Absorbance (450 nm) | Streptavidin Molecules/cm2 |
---|---|---|---|---|
0.0 | 0 | 0 | 0 | 0 |
0.5 | 0.1458 | 1.26 · 1010 | 0.2051 | 7.91 · 1010 |
1.0 | 0.3267 | 2.83 · 1010 | 0.3308 | 1.27 · 1011 |
2.0 | 0.6422 | 5.56 · 1010 | 0.5938 | 2.28 · 1011 |
4.0 | 0.7635 | 6.61 · 1010 | 0.6838 | 2.63 · 1011 |
5.0 | 0.7943 | 6.87 · 1010 | -------- | -------- |
15.0 | 0.8495 | 7.35 · 1010 | 0.8895 | 3.42 · 1011 |
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Marcuello, C.; de Miguel, R.; Lostao, A. Molecular Recognition of Proteins through Quantitative Force Maps at Single Molecule Level. Biomolecules 2022, 12, 594. https://doi.org/10.3390/biom12040594
Marcuello C, de Miguel R, Lostao A. Molecular Recognition of Proteins through Quantitative Force Maps at Single Molecule Level. Biomolecules. 2022; 12(4):594. https://doi.org/10.3390/biom12040594
Chicago/Turabian StyleMarcuello, Carlos, Rocío de Miguel, and Anabel Lostao. 2022. "Molecular Recognition of Proteins through Quantitative Force Maps at Single Molecule Level" Biomolecules 12, no. 4: 594. https://doi.org/10.3390/biom12040594