Structure/Function Studies on the Activation Motif of Two Non-Mammalian Mrap1 Orthologs, and Observations on the Phylogeny of Mrap1, Including a Novel Characterization of an Mrap1 from the Chondrostean Fish, Polyodon spathula
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mc2r and Mrap1 Sequences
2.2. Melanocortin Peptides
2.3. cAMP Reporter Gene Assay
2.4. Statistical Analysis
3. Results
3.1. Analysis of the W18D19Y20I21 Motif in cMRAP1
3.2. Analysis of the Y18D19Y20I21 Motif in bfMrap1
3.3. Pharmacological Properties of Paddlefish Mc2r
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Panel | Transfection with cmc2r | EC50 (M) | p |
---|---|---|---|
2A | cMRAP1 (positive control) | 7.6 × 10−10 ± 2.0 × 10−10 | |
2A | cMRAP1−A18A19A20A21 | n.d. | |
2A | cMRAP1−W18/A | 5.1 × 10−10 ± 12.1 × 10−9 | 0.06 |
2A | cMRAP1−D19/A | 1.6 × 10−9 ± 8.1 × 10−10 | 0.07 |
2B | cMRAP1 (positive control) | 1.6 × 10−10 ± 7.7 × 10−10 | |
2B | cMRAP1−Y21/A | n.d. | |
2B | cMRAP1−I22/A | 1.5 × 10−9 ± 5.2 × 10−10 | 0.11 |
3A | bfmrap1 (positive control) | 3.6 × 10−11 ± 1.1 × 10−10 | |
3A | bfmrap1−a18a19a20a21 | n.d. | |
3A | bfmrap1−y18/a | 3.3 × 10−10 ± 1.3 × 10−10 | 0.07 |
3A | bfmrap1−d19/a | 6.8 × 10−11 ± 4.1 × 10−11 | 0.23 |
3B | bfmrap1 (positive control) | 9.5 × 10−11 ± 3.1 × 10−11 | |
3B | bfmrap1−y20/a | 1.9 × 10−8 ± 4.2 × 10−9 | 0.04 |
3B | bfmrap1−i21/a | 5.8 × 10−10 ± 1.6 × 10−10 | 0.02 |
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Dores, R.M.; McKinley, G.; Meyers, A.; Martin, M.; Shaughnessy, C.A. Structure/Function Studies on the Activation Motif of Two Non-Mammalian Mrap1 Orthologs, and Observations on the Phylogeny of Mrap1, Including a Novel Characterization of an Mrap1 from the Chondrostean Fish, Polyodon spathula. Biomolecules 2022, 12, 1681. https://doi.org/10.3390/biom12111681
Dores RM, McKinley G, Meyers A, Martin M, Shaughnessy CA. Structure/Function Studies on the Activation Motif of Two Non-Mammalian Mrap1 Orthologs, and Observations on the Phylogeny of Mrap1, Including a Novel Characterization of an Mrap1 from the Chondrostean Fish, Polyodon spathula. Biomolecules. 2022; 12(11):1681. https://doi.org/10.3390/biom12111681
Chicago/Turabian StyleDores, Robert M., Greer McKinley, Audrey Meyers, Morgan Martin, and Ciaran A. Shaughnessy. 2022. "Structure/Function Studies on the Activation Motif of Two Non-Mammalian Mrap1 Orthologs, and Observations on the Phylogeny of Mrap1, Including a Novel Characterization of an Mrap1 from the Chondrostean Fish, Polyodon spathula" Biomolecules 12, no. 11: 1681. https://doi.org/10.3390/biom12111681
APA StyleDores, R. M., McKinley, G., Meyers, A., Martin, M., & Shaughnessy, C. A. (2022). Structure/Function Studies on the Activation Motif of Two Non-Mammalian Mrap1 Orthologs, and Observations on the Phylogeny of Mrap1, Including a Novel Characterization of an Mrap1 from the Chondrostean Fish, Polyodon spathula. Biomolecules, 12(11), 1681. https://doi.org/10.3390/biom12111681