Angiotensin II-Induced Vasoconstriction via Rho Kinase Activation in Pressure-Overloaded Rat Thoracic Aortas
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Animals and Abdominal Aortic Banding Operation
2.3. Organ Chamber Experiments
2.4. Western Blotting
2.5. Statistical Analysis
3. Results
3.1. Ang II-Induced Contraction in the Thoracic Aorta of Sham-Operated and Pressure-Overloaded Rats
3.2. Effect of a Rho Kinase Inhibitor on Ang II-Induced Contraction
3.3. Effect of EGFR, Src, or Metalloproteinase Inhibitors on Ang II-Induced Contraction
3.4. Effect of an Erk1/2 Inhibitor on Ang II-Induced Contraction
3.5. Effect of a JAK2 Inhibitor on Ang II-Induced Contraction
3.6. Expression and Phosphorylation of MYPT1, Src, Erk1/2, and JAK2 in Thoracic Aorta
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Product Name | Chemical Name | Concentration Used (µM) |
---|---|---|
AG1478 | 4-(3-chloroanilino)-6,7-dimethoxyquinazoline | 10 |
FR180204 | 5-(2-phenyl-pyrazolo[1,5-a]pyridin-3-yl)-1h-pyrazolo[3,4-c]pyridazin-3-ylamine | 10 |
JAK2 Inhibitor II | 1,2,3,4,5,6-hexabromocyclohexane | 10 |
PP2 | 4-amino-3-(4-chlorophenyl)-1-(t-butyl)-1h-pyrazolo[3,4-d]pyrimidine | 3 |
TAPI-0 | n-(R)-(2-[hydroxyaminocarbonyl]methyl)-4-methylpentanoyl-l-naphthylalanyl-l-alanine amide | 10 |
Y-27632 | R-(+)-trans-n-(4-pyridyl)-4-(1-aminoethyl)-cyclohexanecarboxamide | 10 |
Antibody | Dilution | Catalog No. | Purchased from |
---|---|---|---|
MYPT1 | 1:600 | #2634 | Cell Signaling Technology (Danvers, MA, USA) |
Thr-853-phosphorylated MYPT1 | 1:250 | CSB-PA020015 | CUSABIO (Houston, TX, USA) |
Erk1/2 | 1:5000 | #4695 | Cell Signaling Technology (Danvers, MA, USA) |
Thr-202/Tyr-204-phosphorylated Erk1/2 | 1:5000 | #9101 | Cell Signaling Technology (Danvers, MA, USA) |
JAK2 | 1:500 | #3230 | Cell Signaling Technology (Danvers, MA, USA) |
Tyr-1008-phosphorylated JAK2 | 1:200 | #8082 | Cell Signaling Technology (Danvers, MA, USA) |
Src | 1:750 | #2102 | Cell Signaling Technology (Danvers, MA, USA) |
Tyr-416-phosphorylated Src | 1:750 | #2101 | Cell Signaling Technology (Danvers, MA, USA) |
β-actin | 1:5000 | A5441 | Sigma-Aldrich (St. Louis, MO, USA) |
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Terada, Y.; Yayama, K. Angiotensin II-Induced Vasoconstriction via Rho Kinase Activation in Pressure-Overloaded Rat Thoracic Aortas. Biomolecules 2021, 11, 1076. https://doi.org/10.3390/biom11081076
Terada Y, Yayama K. Angiotensin II-Induced Vasoconstriction via Rho Kinase Activation in Pressure-Overloaded Rat Thoracic Aortas. Biomolecules. 2021; 11(8):1076. https://doi.org/10.3390/biom11081076
Chicago/Turabian StyleTerada, Yuka, and Katsutoshi Yayama. 2021. "Angiotensin II-Induced Vasoconstriction via Rho Kinase Activation in Pressure-Overloaded Rat Thoracic Aortas" Biomolecules 11, no. 8: 1076. https://doi.org/10.3390/biom11081076