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Influence of Dopamine on Fluorescent Advanced Glycation End Products Formation Using Drosophila melanogaster

1
Department of Biotechnology, University of Rijeka, 51000 Rijeka, Croatia
2
Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey GU2 7XH, UK
3
High School Andrija Mohorovičić, 51000 Rijeka, Croatia
*
Author to whom correspondence should be addressed.
Biomolecules 2021, 11(3), 453; https://doi.org/10.3390/biom11030453
Received: 12 February 2021 / Revised: 9 March 2021 / Accepted: 13 March 2021 / Published: 17 March 2021
(This article belongs to the Section Chemical Biology)
Non-enzymatic glycation and covalent modification of proteins leads to Advanced Glycation End products (AGEs). AGEs are biomarkers of aging and neurodegenerative disease, and can be induced by impaired neuronal signaling. The objective of this study was to investigate if manipulation of dopamine (DA) in vitro using the model protein, bovine serum albumin (BSA), and in vivo using the model organism Drosophila melanogaster, influences fluorescent AGEs (fAGEs) formation as an indicator of dopamine-induced oxidation events. DA inhibited fAGEs-BSA synthesis in vitro, suggesting an anti-oxidative effect, which was not observed when flies were fed DA. Feeding flies cocaine and methamphetamine led to increased fAGEs formation. Mutants lacking the dopaminergic transporter or the D1-type showed further elevation of fAGEs accumulation, indicating that the long-term perturbation in DA function leads to higher production of fAGEs. To confirm that DA has oxidative properties in vivo, we fed flies antioxidant quercetin (QUE) together with methamphetamine. QUE significantly decreased methamphetamine-induced fAGEs formation suggesting that the perturbation of DA function in vivo leads to increased oxidation. These findings present arguments for the use of fAGEs as a biomarker of DA-associated neurodegenerative changes and for assessment of antioxidant interventions such as QUE treatment. View Full-Text
Keywords: dopamine (DA); fluorescent advanced glycation end products (fAGEs); psychostimulants; oxidation; Drosophila melanogaster dopamine (DA); fluorescent advanced glycation end products (fAGEs); psychostimulants; oxidation; Drosophila melanogaster
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MDPI and ACS Style

Filošević Vujnović, A.; Jović, K.; Pištan, E.; Andretić Waldowski, R. Influence of Dopamine on Fluorescent Advanced Glycation End Products Formation Using Drosophila melanogaster. Biomolecules 2021, 11, 453. https://doi.org/10.3390/biom11030453

AMA Style

Filošević Vujnović A, Jović K, Pištan E, Andretić Waldowski R. Influence of Dopamine on Fluorescent Advanced Glycation End Products Formation Using Drosophila melanogaster. Biomolecules. 2021; 11(3):453. https://doi.org/10.3390/biom11030453

Chicago/Turabian Style

Filošević Vujnović, Ana, Katarina Jović, Emanuel Pištan, and Rozi Andretić Waldowski. 2021. "Influence of Dopamine on Fluorescent Advanced Glycation End Products Formation Using Drosophila melanogaster" Biomolecules 11, no. 3: 453. https://doi.org/10.3390/biom11030453

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