Next Article in Journal
New Triterpene Glycosides from the Far Eastern Starfish Solaster pacificus and Their Biological Activity
Previous Article in Journal
COVID-19 Rapid Antigen Test as Screening Strategy at Points of Entry: Experience in Lazio Region, Central Italy, August–October 2020
 
 
Article

Characterization of the Lipidome and Biophysical Properties of Membranes from High Five Insect Cells Expressing Mouse P-Glycoprotein

1
Coimbra Chemistry Center, Chemistry Department, FCTUC, University of Coimbra, 3004-535 Coimbra, Portugal
2
CNC—Center for Neuroscience and Cell Biology, University of Coimbra, 3004-535 Coimbra, Portugal
3
Laboratory of Cell Biology, CCR, National Cancer Institute, NIH, Bethesda, MD 20892, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Mikhail Bogdanov
Biomolecules 2021, 11(3), 426; https://doi.org/10.3390/biom11030426
Received: 5 February 2021 / Revised: 11 March 2021 / Accepted: 12 March 2021 / Published: 14 March 2021
The lipid composition of biomembranes influences the properties of the lipid bilayer and that of the proteins. In this study, the lipidome and the lipid/protein ratio of membranes from High Five™ insect cells overexpressing mouse P-glycoprotein was characterized. This provides a better understanding of the lipid environment in which P-glycoprotein is embedded, and thus of its functional and structural properties. The relative abundance of the distinct phospholipid classes and their acyl chain composition was characterized. A mass ratio of 0.57 ± 0.11 phospholipids to protein was obtained. Phosphatidylethanolamines are the most abundant phospholipids, followed by phosphatidylcholines. Membranes are also enriched in negatively charged lipids (phosphatidylserines, phosphatidylinositols and phosphatidylglycerols), and contain small amounts of sphingomyelins, ceramides and monoglycosilatedceramides. The most abundant acyl chains are monounsaturated, with significant amounts of saturated chains. The characterization of the phospholipids by HPLC-MS allowed identification of the combination of acyl chains, with palmitoyl-oleoyl being the most representative for all major phospholipid classes except for phosphatidylserines, which are mostly saturated. A mixture of POPE:POPC:POPS in the ratio 45:35:20 is proposed for the preparation of simple representative model membranes. The adequacy of the model membranes was further evaluated by characterizing their surface potential and fluidity. View Full-Text
Keywords: lipidome; High Five insect cells; membrane proteins; biomembranes lipidome; High Five insect cells; membrane proteins; biomembranes
Show Figures

Figure 1

MDPI and ACS Style

Moreno, M.J.; Teles Martins, P.A.; Bernardino, E.F.; Abel, B.; Ambudkar, S.V. Characterization of the Lipidome and Biophysical Properties of Membranes from High Five Insect Cells Expressing Mouse P-Glycoprotein. Biomolecules 2021, 11, 426. https://doi.org/10.3390/biom11030426

AMA Style

Moreno MJ, Teles Martins PA, Bernardino EF, Abel B, Ambudkar SV. Characterization of the Lipidome and Biophysical Properties of Membranes from High Five Insect Cells Expressing Mouse P-Glycoprotein. Biomolecules. 2021; 11(3):426. https://doi.org/10.3390/biom11030426

Chicago/Turabian Style

Moreno, Maria João, Patrícia Alexandra Teles Martins, Eva F. Bernardino, Biebele Abel, and Suresh V. Ambudkar. 2021. "Characterization of the Lipidome and Biophysical Properties of Membranes from High Five Insect Cells Expressing Mouse P-Glycoprotein" Biomolecules 11, no. 3: 426. https://doi.org/10.3390/biom11030426

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop