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Article

Microwave-Assisted Rapid Green Synthesis of Gold Nanoparticles Using Seed Extract of Trachyspermum ammi: ROS Mediated Biofilm Inhibition and Anticancer Activity

1
Department of Botany and Microbiology, College of Science, King Saud University, 2460, Riyadh 11451, Saudi Arabia
2
Department of Food Science and Nutrition, College of Food and Agriculture Sciences, King Saud University, 2460, Riyadh 11451, Saudi Arabia
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Department of Ag. Microbiology, Aligarh Muslim University, Aligarh 202002, India
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Central Laboratory, Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, 2460, Riyadh 11451, Saudi Arabia
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Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, 2460, Riyadh 11451, Saudi Arabia
6
Department of Biology, College of Science and Humanities, Prince Sattam bin Abdulaziz University, Alkharj 11942, Saudi Arabia
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Bilal Haider Abbasi
Biomolecules 2021, 11(2), 197; https://doi.org/10.3390/biom11020197
Received: 31 December 2020 / Revised: 17 January 2021 / Accepted: 19 January 2021 / Published: 30 January 2021
Green synthesis of metal nanoparticles using plant extracts as capping and reducing agents for the biomedical applications has received considerable attention. Moreover, emergence and spread of multidrug resistance among bacterial pathogens has become a major health concern and lookout for novel alternative effective drugs has gained momentum. In current study, we synthesized gold nanoparticles using the seed extract of Trachyspermum ammi (TA-AuNPs), assessed its efficacy against drug resistant biofilms of Listeria monocytogenes and Serratia marcescens, and evaluated its anticancer potential against HepG2 cancer cell lines. Microwave-assisted green synthesis of gold nanoparticles was carried out and characterization was done using UV-vis spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), and dynamic light scattering (DLS). Most nanoparticles were observed as spherical and spheroidal with few anisotropies with an average crystalline size of 16.63 nm. Synthesized TA-AuNPs demonstrated significant biofilm inhibitory activity against L. monocytogenes (73%) as well as S. marcescens (81%). Exopolysaccharide (EPS), motility, and CSH, key elements that facilitate the formation and maintenance of biofilm were also inhibited significantly at the tested sub-minimum inhibitory concentrations (sub-MICs). Further, TA-AuNPs effectively obliterated preformed mature biofilms of S. marcescens and L. monocytogenes by 64% and 58%, respectively. Induction of intracellular ROS production in TA-AuNPs treated bacterial cells could be the plausible mechanism for the reduced biofilm formation in test pathogens. Administration of TA-AuNPs resulted in the arrest of cellular proliferation in a concentration-dependent manner. TA-AuNPs decrease the intracellular GSH in HepG2 cancer cell lines, cells become more prone to ROS generation, hence induce apoptosis. Thus, this work proposes a new eco-friendly and rapid approach for fabricating NPs which can be exploited for multifarious biomedical applications. View Full-Text
Keywords: gold nanoparticles; green synthesis; Trachyspermum ammi; antibiofilm; anticancer; HepG2 cell lines gold nanoparticles; green synthesis; Trachyspermum ammi; antibiofilm; anticancer; HepG2 cell lines
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MDPI and ACS Style

Perveen, K.; Husain, F.M.; Qais, F.A.; Khan, A.; Razak, S.; Afsar, T.; Alam, P.; Almajwal, A.M.; Abulmeaty, M.M.A. Microwave-Assisted Rapid Green Synthesis of Gold Nanoparticles Using Seed Extract of Trachyspermum ammi: ROS Mediated Biofilm Inhibition and Anticancer Activity. Biomolecules 2021, 11, 197. https://doi.org/10.3390/biom11020197

AMA Style

Perveen K, Husain FM, Qais FA, Khan A, Razak S, Afsar T, Alam P, Almajwal AM, Abulmeaty MMA. Microwave-Assisted Rapid Green Synthesis of Gold Nanoparticles Using Seed Extract of Trachyspermum ammi: ROS Mediated Biofilm Inhibition and Anticancer Activity. Biomolecules. 2021; 11(2):197. https://doi.org/10.3390/biom11020197

Chicago/Turabian Style

Perveen, Kahkashan, Fohad M. Husain, Faizan A. Qais, Altaf Khan, Suhail Razak, Tayyaba Afsar, Pravej Alam, Ali M. Almajwal, and Mahmoud M.A. Abulmeaty 2021. "Microwave-Assisted Rapid Green Synthesis of Gold Nanoparticles Using Seed Extract of Trachyspermum ammi: ROS Mediated Biofilm Inhibition and Anticancer Activity" Biomolecules 11, no. 2: 197. https://doi.org/10.3390/biom11020197

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