The Odorant Binding Protein, SiOBP5, Mediates Alarm Pheromone Olfactory Recognition in the Red Imported Fire Ant, Solenopsis invicta
Abstract
:1. Introduction
2. Materials and Methods
2.1. Insects
2.2. Chemical
2.3. Total RNA Extraction, cDNA and dsRNA Synthesis
2.4. dsRNA Injection and Feeding
2.5. Electroantennography (EAG) Assays
2.6. Real-Time Quantitative RT-PCR
2.7. Statistical Analysis
3. Results
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Primer Use | Sequence (5′–3′) |
---|---|---|
GFP | dsRNA synthesis | T7F: TAATACGACTCACTATAGGG CCATCCTGGTCGAGCTGGACGGCG |
T7R:TAATACGACTCACTATAGGG TCCAGCAGGACCATGTGATCGCGC | ||
OBP1 | dsRNA synthesis | T7F: TAATACGACTCACTATAGGG AGTGTTGCTACAGGCGATTTA |
T7R: TAATACGACTCACTATAGGG CTTGTACGGCGCATTTGTTC | ||
OBP5 | dsRNA synthesis | T7F: TAATACGACTCACTATAGGG ATGTGCGCGCGTTTACTA |
T7R: TAATACGACTCACTATAGGG AAAGGACCCGCCATTTCA | ||
OBP6 | dsRNA synthesis | T7F: TAATACGACTCACTATAGGG TCGCTTGTGTTTATGGCAAATC |
T7R: TAATACGACTCACTATAGGG TATGCTAACGCACACCCTTC | ||
OrCo | dsRNA synthesis | T7F: TAATACGACTCACTATAGGG GTTTCGCGCTTCTACTCCAC |
T7R: TAATACGACTCACTATAGGG CTCCGATCCATCGTACCAGT | ||
OrCo | qRT-PCR | F: GGTCCGATCGTTCTATCCATTC R: CCAACGAATTGGCGTTGATAAG |
GAPDH | qRT-PCR | F: AAGCTGTGGCGTGATGGCG R: AGGAGGCAGGCTTGGCGAG |
GFP | qRT-PCR | F: AAGCTGTGGCGTGATGGCCG R: AGGAGGCAGGCTTGGCGAGT |
OBP1 | qRT-PCR | F: CGATGGGAAACTCTCGAATGA R: CAGCATCTCAGCTTCCAAATTAC |
OBP5 | qRT-PCR | F: GGACGCATAGATGACGGTATG R: TGAGCAATCATCGCCAGTAG |
OBP6 | qRT-PCR | F: CGCTTGTGTTTATGGCAAATCT R: TTTGATCCTTGCCCACTCTATC |
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Du, Y.; Chen, J. The Odorant Binding Protein, SiOBP5, Mediates Alarm Pheromone Olfactory Recognition in the Red Imported Fire Ant, Solenopsis invicta. Biomolecules 2021, 11, 1595. https://doi.org/10.3390/biom11111595
Du Y, Chen J. The Odorant Binding Protein, SiOBP5, Mediates Alarm Pheromone Olfactory Recognition in the Red Imported Fire Ant, Solenopsis invicta. Biomolecules. 2021; 11(11):1595. https://doi.org/10.3390/biom11111595
Chicago/Turabian StyleDu, Yuzhe, and Jian Chen. 2021. "The Odorant Binding Protein, SiOBP5, Mediates Alarm Pheromone Olfactory Recognition in the Red Imported Fire Ant, Solenopsis invicta" Biomolecules 11, no. 11: 1595. https://doi.org/10.3390/biom11111595