RNA Metabolism Guided by RNA Modifications: The Role of SMUG1 in rRNA Quality Control
1
Department of Clinical Molecular Biology, University of Oslo, 0318 Oslo, Norway
2
Department of Clinical Molecular Biology (EpiGen), Akershus University Hospital, 1478 Lørenskog, Norway
3
Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA 92093, USA
4
Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, NTNU, NO-7491 Trondheim, Norway
5
SINTEF Ocean AS, 7010 Trondheim, Norway
*
Author to whom correspondence should be addressed.
Biomolecules 2021, 11(1), 76; https://doi.org/10.3390/biom11010076
Received: 23 November 2020
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Revised: 29 December 2020
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Accepted: 5 January 2021
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Published: 8 January 2021
(This article belongs to the Section Molecular Biology)
RNA modifications are essential for proper RNA processing, quality control, and maturation steps. In the last decade, some eukaryotic DNA repair enzymes have been shown to have an ability to recognize and process modified RNA substrates and thereby contribute to RNA surveillance. Single-strand-selective monofunctional uracil-DNA glycosylase 1 (SMUG1) is a base excision repair enzyme that not only recognizes and removes uracil and oxidized pyrimidines from DNA but is also able to process modified RNA substrates. SMUG1 interacts with the pseudouridine synthase dyskerin (DKC1), an enzyme essential for the correct assembly of small nucleolar ribonucleoproteins (snRNPs) and ribosomal RNA (rRNA) processing. Here, we review rRNA modifications and RNA quality control mechanisms in general and discuss the specific function of SMUG1 in rRNA metabolism. Cells lacking SMUG1 have elevated levels of immature rRNA molecules and accumulation of 5-hydroxymethyluridine (5hmU) in mature rRNA. SMUG1 may be required for post-transcriptional regulation and quality control of rRNAs, partly by regulating rRNA and stability.
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Keywords:
SMUG1; rRNA processing; modified bases
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MDPI and ACS Style
Lirussi, L.; Demir, Ö.; You, P.; Sarno, A.; Amaro, R.E.; Nilsen, H. RNA Metabolism Guided by RNA Modifications: The Role of SMUG1 in rRNA Quality Control. Biomolecules 2021, 11, 76. https://doi.org/10.3390/biom11010076
AMA Style
Lirussi L, Demir Ö, You P, Sarno A, Amaro RE, Nilsen H. RNA Metabolism Guided by RNA Modifications: The Role of SMUG1 in rRNA Quality Control. Biomolecules. 2021; 11(1):76. https://doi.org/10.3390/biom11010076
Chicago/Turabian StyleLirussi, Lisa, Özlem Demir, Panpan You, Antonio Sarno, Rommie E. Amaro, and Hilde Nilsen. 2021. "RNA Metabolism Guided by RNA Modifications: The Role of SMUG1 in rRNA Quality Control" Biomolecules 11, no. 1: 76. https://doi.org/10.3390/biom11010076
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