Next Article in Journal
The Gintonin-Enriched Fraction of Ginseng Regulates Lipid Metabolism and Browning via the cAMP-Protein Kinase a Signaling Pathway in Mice White Adipocytes
Next Article in Special Issue
Biochemical Composition of Cumin Seeds, and Biorefining Study
Previous Article in Journal
ProAKAP4 as Novel Molecular Marker of Sperm Quality in Ram: An Integrative Study in Fresh, Cooled and Cryopreserved Sperm
Previous Article in Special Issue
Reduce, Reuse and Recycle in Protein Chromatography: Development of an Affinity Adsorbent from Waste Paper and Its Application for the Purification of Proteases from Fish By-Products
Article

Subcritical Methanol Extraction of the Stone of Japanese Apricot Prunus mume Sieb. et Zucc.

1
Faculty of Biology-oriented Science and Technology, Kindai University, Kinokawa, Wakayama 649-6493, Japan
2
School of Medicine, Department of Chemistry, Wakayama Medical University, Wakayama 641-0011, Japan
3
Industrial Technology Center of Wakayama Prefecture, Wakayama 649-6261, Japan
4
Faculty of Education, Wakayama University, Wakayama 640-8510, Japan
5
Center of Regional Revitalization, Research Center for Food and Agriculture, Wakayama University, Wakayama 640-8510, Japan
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(7), 1047; https://doi.org/10.3390/biom10071047
Received: 28 May 2020 / Revised: 11 July 2020 / Accepted: 13 July 2020 / Published: 15 July 2020
The pits of Japanese apricot, Prunus mume Sieb. et Zucc., which are composed of stones, husks, kernels, and seeds, are unused by-products of the processing industry in Japan. The processing of Japanese apricot fruits generates huge amounts of waste pits, which are disposed of in landfills or, to a lesser extent, burned to form charcoal. Mume stones mainly consist of cellulose, hemicellulose, and lignin. Herein, we attempted to solubilize the wood-like carapace (stone) encasing the pit by subcritical fluid extraction with the aim of extracting useful chemicals. The characteristics of the main phenolic constituents were elucidated by liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR) analyses. The degrees of solubility for various treatments (190 °C; 3 h) were determined as follows: subcritical water (54.9%), subcritical 50% methanol (65.5%), subcritical 90% methanol (37.6%), subcritical methanol (23.6%), and subcritical isopropyl alcohol (14.4%). Syringaldehyde, sinapyl alcohol, coniferyl alcohol methyl ether, sinapyl alcohol methyl ether, 5-(hydroxymethyl)-2-furfural, and furfural were present in the subcritical 90% methanol extract. Coniferyl and sinapyl alcohols (monolignols) are source materials for the biosynthesis of lignin, and syringaldehyde occur in trace amounts in wood. Our current findings provide a solubilization method that allows the main phenolic constituents of the pits to be extracted under mild conditions. This technique for obtaining subcritical extracts shows great potential for further applications. View Full-Text
Keywords: Prunus mume; Japanese apricot; mume stones; subcritical methanol extraction; coniferyl alcohol; sinapyl alcohol Prunus mume; Japanese apricot; mume stones; subcritical methanol extraction; coniferyl alcohol; sinapyl alcohol
Show Figures

Figure 1

MDPI and ACS Style

Kawabata, T.; Tanaka, Y.; Horinishi, A.; Mori, M.; Hosoda, A.; Yamamoto, N.; Mitani, T. Subcritical Methanol Extraction of the Stone of Japanese Apricot Prunus mume Sieb. et Zucc. Biomolecules 2020, 10, 1047. https://doi.org/10.3390/biom10071047

AMA Style

Kawabata T, Tanaka Y, Horinishi A, Mori M, Hosoda A, Yamamoto N, Mitani T. Subcritical Methanol Extraction of the Stone of Japanese Apricot Prunus mume Sieb. et Zucc. Biomolecules. 2020; 10(7):1047. https://doi.org/10.3390/biom10071047

Chicago/Turabian Style

Kawabata, Tomoaki, Yoshie Tanaka, Asako Horinishi, Megumi Mori, Asao Hosoda, Nami Yamamoto, and Takahiko Mitani. 2020. "Subcritical Methanol Extraction of the Stone of Japanese Apricot Prunus mume Sieb. et Zucc." Biomolecules 10, no. 7: 1047. https://doi.org/10.3390/biom10071047

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop