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Open AccessArticle

Fluorescein Derivatives as Antibacterial Agents Acting via Membrane Depolarization

1
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119991, Russia
2
Department of Biochemistry, University of Maastricht, Cardiovascular Research Institute Maastricht, Universiteitssingel 50, 6229 ER, Maastricht, The Netherlands
3
Center of Life Sciences, Skolkovo Institute of Science and Technology, Moscow 121205, Russia
*
Authors to whom correspondence should be addressed.
Biomolecules 2020, 10(2), 309; https://doi.org/10.3390/biom10020309 (registering DOI)
Received: 23 December 2019 / Revised: 3 February 2020 / Accepted: 13 February 2020 / Published: 15 February 2020
Appending a lipophylic alkyl chain by ester bond to fluorescein has been previously shown to convert this popular dye into an effective protonophoric uncoupler of oxidative phosphorylation in mitochondria, exhibiting neuro- and nephroprotective effects in murine models. In line with this finding, we here report data on the pronounced depolarizing effect of a series of fluorescein decyl esters on bacterial cells. The binding of the fluorescein derivatives to Bacillus subtilis cells was monitored by fluorescence microscopy and fluorescence correlation spectroscopy (FCS). FCS revealed the energy-dependent accumulation of the fluorescein esters with decyl(triphenyl)- and decyl(tri-p-tolyl)phosphonium cations in the bacterial cells. The latter compound proved to be the most potent in suppressing B. subtilis growth.
Keywords: fluorescein derivative; antibacterial agent; bacterial membrane depolarization; mitochondria; membrane potential; fluorescent uncoupler fluorescein derivative; antibacterial agent; bacterial membrane depolarization; mitochondria; membrane potential; fluorescent uncoupler
MDPI and ACS Style

Nazarov, P.A.; Kirsanov, R.S.; Denisov, S.S.; Khailova, L.S.; Karakozova, M.V.; Lyamzaev, K.G.; Korshunova, G.A.; Lukyanov, K.A.; Kotova, E.A.; Antonenko, Y.N. Fluorescein Derivatives as Antibacterial Agents Acting via Membrane Depolarization. Biomolecules 2020, 10, 309.

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