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Open AccessArticle

Modified Potentiometric Screen-Printed Electrodes Based on Imprinting Character for Sodium Deoxycholate Determination

1
Department of Chemistry, Faculty of Science, Ain Shams University, Cairo 11566, Egypt
2
Chemistry Department, College for Women, Ain Shams University, Heliopolis, Cairo 11751, Egypt
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Pharmaceutical Chemistry Department, Drug Exploration & Development Chair (DEDC), College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
4
Applied Organic Chemistry Department, National Research Center, Giza 12622, Egypt
*
Authors to whom correspondence should be addressed.
Biomolecules 2020, 10(2), 251; https://doi.org/10.3390/biom10020251
Received: 12 January 2020 / Revised: 4 February 2020 / Accepted: 4 February 2020 / Published: 6 February 2020
(This article belongs to the Section Biochemistry)
Potentiometric sensors have a great influence on the determination of most various compounds in their matrices. Therefore, efficient and new sensors were introduced to measure sodium Deoxycholate (NaDC) as a bile acid salt. These sensors are based on NaDC imprinted polymer (MIP) as sensory element. The MIP beads were synthesized using thermal polymerization pathway, in which acrylamide (AAm), ethylene glycol dimethacrylate (EGDMA), NaDC, and benzoyl peroxide (BPO) were used as the functional monomer, cross-linker, template, and initiator, respectively. The proposed sensors were fabricated using a coated screen-printed platform and the sensing membrane was modified by single-walled carbon nanotubes (SWCNTs) as an ion-to-electron transducer. The sensors exhibited high sensitivity that reached 4.7 × 10−5 M of near-Nernestian slope (−60.1 ± 0.9 mV/decade, r2 = 0.999 (n= 5)). In addition, the sensors revealed high selectivity, long lifetime, high potential stability, and conductivity that ensure reproducible and accurate results over a long time. MIP characterization was performed using Fourier Transform-Infrared (FT-IR) and a scanning electron microscope (SEM). Regarding the interaction of NaDC with serum albumin (SA), albumin is determined in human serum samples as human serum albumin (HSA), which was collected from different volunteers of different ages and gender.
Keywords: sodium deoxycholate (NaDC); molecular imprinted polymer (MIP); screen-printed ion selective electrodes; single-walled carbon nanotubes (SWCNTs); human serum albumin (HSA) sodium deoxycholate (NaDC); molecular imprinted polymer (MIP); screen-printed ion selective electrodes; single-walled carbon nanotubes (SWCNTs); human serum albumin (HSA)
MDPI and ACS Style

H. Kamel, A.; Ezzat, S.; Ahmed, M.A.; Amr, A.-G.; A. Almehizia, A.; A. Al-Omar, M. Modified Potentiometric Screen-Printed Electrodes Based on Imprinting Character for Sodium Deoxycholate Determination. Biomolecules 2020, 10, 251.

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