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Open AccessArticle

Differential Metabolic Reprogramming in Paenibacillus alvei-Primed Sorghum bicolor Seedlings in Response to Fusarium pseudograminearum Infection

1
Department of Plant and Soil Sciences, Faculty of Plant Pathology, University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028, South Africa
2
Centre for Plant Metabolomics Research, Department of Biochemistry, Faculty of Science, University of Johannesburg, P.O. Box 524, Auckland Park, Johannesburg 2006, South Africa
*
Author to whom correspondence should be addressed.
Metabolites 2019, 9(7), 150; https://doi.org/10.3390/metabo9070150
Received: 25 April 2019 / Revised: 7 June 2019 / Accepted: 10 July 2019 / Published: 23 July 2019
(This article belongs to the Special Issue Metabolomics in Agriculture)
Metabolic changes in sorghum seedlings in response to Paenibacillus alvei (NAS-6G6)-induced systemic resistance against Fusarium pseudograminearum crown rot were investigated by means of untargeted ultra-high performance liquid chromatography-high definition mass spectrometry (UHPLC-HDMS). Treatment of seedlings with the plant growth-promoting rhizobacterium P. alvei at a concentration of 1 × 108 colony forming units mL−1 prior to inoculation with F. pseudograminearum lowered crown rot disease severity significantly at the highest inoculum dose of 1 × 106 spores mL−1. Intracellular metabolites were subsequently methanol-extracted from treated and untreated sorghum roots, stems and leaves at 1, 4 and 7 days post inoculation (d.p.i.) with F. pseudograminearum. The extracts were analysed on an UHPLC-HDMS platform, and the data chemometrically processed to determine metabolic profiles and signatures related to priming and induced resistance. Significant treatment-related differences in primary and secondary metabolism post inoculation with F. pseudograminearum were observed between P. alvei-primed versus naïve S. bicolor seedlings. The differential metabolic reprogramming in primed plants comprised of a quicker and/or enhanced upregulation of amino acid-, phytohormone-, phenylpropanoid-, flavonoid- and lipid metabolites in response to inoculation with F. pseudograminearum. View Full-Text
Keywords: crown rot; Fusarium pseudograminearum; induced systemic resistance; LC-MS; Paenibacillus alvei; PGPR; phytoalexin; priming; Sorghum bicolor crown rot; Fusarium pseudograminearum; induced systemic resistance; LC-MS; Paenibacillus alvei; PGPR; phytoalexin; priming; Sorghum bicolor
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MDPI and ACS Style

Carlson, R.; Tugizimana, F.; Steenkamp, P.A.; Dubery, I.A.; Labuschagne, N. Differential Metabolic Reprogramming in Paenibacillus alvei-Primed Sorghum bicolor Seedlings in Response to Fusarium pseudograminearum Infection. Metabolites 2019, 9, 150.

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