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An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells

Centre for Analysis and Synthesis, Department of Chemistry, Lund University, 223 62 Lund, Sweden
Laboratory Genetic Metabolic Diseases, Department of Clinical Chemistry, Amsterdam UMC, Location AMC, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands
Author to whom correspondence should be addressed.
Metabolites 2019, 9(3), 45;
Received: 21 January 2019 / Revised: 15 February 2019 / Accepted: 28 February 2019 / Published: 5 March 2019
PDF [2020 KB, uploaded 11 March 2019]


Oxidative stress plays a role in the onset and progression of a number of diseases, such as Alzheimer’s disease, diabetes and cancer, as well as ageing. Oxidative stress is caused by an increased production of reactive oxygen species and reduced antioxidant activity, resulting in the oxidation of glutathione. The ratio of reduced to oxidised glutathione is often used as a marker of the redox state in the cell. Whereas a variety of methods have been developed to measure glutathione in blood samples, methods to measure glutathione in cultured cells are scarce. Here we present a protocol to measure glutathione levels in cultured human and yeast cells using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS). View Full-Text
Keywords: oxidative stress; glutathione; cultured cells; yeast; mass spectrometry oxidative stress; glutathione; cultured cells; yeast; mass spectrometry

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Herzog, K.; IJlst, L.; van Cruchten, A.G.; van Roermund, C.W.; Kulik, W.; Wanders, R.J.A.; Waterham, H.R. An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells. Metabolites 2019, 9, 45.

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