An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells
AbstractOxidative stress plays a role in the onset and progression of a number of diseases, such as Alzheimer’s disease, diabetes and cancer, as well as ageing. Oxidative stress is caused by an increased production of reactive oxygen species and reduced antioxidant activity, resulting in the oxidation of glutathione. The ratio of reduced to oxidised glutathione is often used as a marker of the redox state in the cell. Whereas a variety of methods have been developed to measure glutathione in blood samples, methods to measure glutathione in cultured cells are scarce. Here we present a protocol to measure glutathione levels in cultured human and yeast cells using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS). View Full-Text
- Supplementary File 1:
Supplementary (PDF, 177 KB)
Share & Cite This Article
Herzog, K.; IJlst, L.; van Cruchten, A.G.; van Roermund, C.W.; Kulik, W.; Wanders, R.J.A.; Waterham, H.R. An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells. Metabolites 2019, 9, 45.
Herzog K, IJlst L, van Cruchten AG, van Roermund CW, Kulik W, Wanders RJA, Waterham HR. An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells. Metabolites. 2019; 9(3):45.Chicago/Turabian Style
Herzog, Katharina; IJlst, Lodewijk; van Cruchten, Arno G.; van Roermund, Carlo W.; Kulik, Wim; Wanders, Ronald J.A.; Waterham, Hans R. 2019. "An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells." Metabolites 9, no. 3: 45.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.