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Open AccessArticle

Soluble Sugar and Lipid Readjustments in the Yarrowia lipolytica Yeast at Various Temperatures and pH

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A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, bld 33-2, Leninsly Prospect, Moscow 119071, Russia
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Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave. 33/2, Moscow 119071, Russia
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Authors to whom correspondence should be addressed.
Metabolites 2019, 9(12), 307; https://doi.org/10.3390/metabo9120307
Received: 25 November 2019 / Revised: 13 December 2019 / Accepted: 15 December 2019 / Published: 17 December 2019
(This article belongs to the Special Issue Metabolomics in Yeast and Fermentation)
Microorganisms cope with a wide range of environmental challenges using different mechanisms. Their ability to prosper at extreme ambient pH and high temperatures has been well reported, but the adaptation mechanism often remains unrevealed. In this study, we addressed the dynamics of lipid and sugar profiles upon different cultivation conditions. The results showed that the cells grown at various pH and optimal temperature contained mannitol as the major cytosol sugar alcohol. The elevated temperature of 38 °C led to a two- to three-fold increase in total cytosol sugars with concurrent substitution of mannitol for trehalose. Lipid composition in the cells at optimal temperature changed insignificantly at any pH tested. The increase in the temperature caused some drop in the storage and membrane lipid levels, remarkable changes in their composition, and the degree of unsaturated fatty acids. It was shown that the fatty acid composition of some membrane phospholipids varied considerably at changing pH and temperature values. The data showed a pivotal role and flexibility of the sugar and lipid composition of Y. lipolytica W29 in adaptation to unfavorable environmental conditions. View Full-Text
Keywords: thermal shock; ambient pH; yeast; metabolic readjustments; lipids; carbohydrates thermal shock; ambient pH; yeast; metabolic readjustments; lipids; carbohydrates
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Sekova, V.Y.; Dergacheva, D.I.; Isakova, E.P.; Gessler, N.N.; Tereshina, V.M.; Deryabina, Y.I. Soluble Sugar and Lipid Readjustments in the Yarrowia lipolytica Yeast at Various Temperatures and pH. Metabolites 2019, 9, 307.

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