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Open AccessArticle

Relationship between Metabolomics Profile of Perilymph in Cochlear-Implanted Patients and Duration of Hearing Loss

1
Service ORL et Chirurgie Cervico-Faciale, CHRU de Tours, 37000 Tours, France
2
Laboratoire de Biochimie et Biologie Moléculaire, CHRU de Tours, 37000 Tours, France
3
Université François-Rabelais, 37000 Tours, France
4
Inserm U1253, 37000 Tours, France
5
Centre SLA, Service de Neurologie, CHRU Bretonneau, 37044 Tours, France
6
PPF (Programme Pluri-Formation), Université François-Rabelais, 37000 Tours, France
*
Author to whom correspondence should be addressed.
Metabolites 2019, 9(11), 262; https://doi.org/10.3390/metabo9110262
Received: 27 August 2019 / Revised: 28 October 2019 / Accepted: 30 October 2019 / Published: 1 November 2019
Perilymph metabolomic analysis is an emerging innovative strategy to improve our knowledge of physiopathology in sensorineural hearing loss. This study aims to develop a metabolomic profile of human perilymph with which to evaluate the relationship between metabolome and the duration of hearing loss. Inclusion criteria were eligibility for cochlear implantation and easy access to the round window during surgery; patients with residual acoustic hearing in the ear to be implanted were excluded. Human perilymph was sampled from 19 subjects during cochlear implantation surgery. The perilymph analysis was performed by Liquid Chromatography−High-Resolution Mass and data were analyzed by supervised multivariate analysis based on Partial Least-Squares Discriminant Analysis and univariate analysis. Samples were grouped according to their median duration of hearing loss. We included the age of patients as a covariate in our models. Statistical analysis and pathways evaluation were performed using Metaboanalyst. Nineteen samples of human perilymph were analyzed, and a total of 106 different metabolites were identified. Metabolomic profiles were significantly different for subjects with ≤12 or >12 years of hearing loss, highlighting the following discriminant compounds: N-acetylneuraminate, glutaric acid, cystine, 2-methylpropanoate, butanoate and xanthine. As expected, the age of patients was also one of the main discriminant parameters. Metabolic signatures were observed for duration of hearing loss. These findings are promising steps towards illuminating the pathophysiological pathways associated with etiologies of sensorineural hearing loss, and hold open the possibilities of further explorations into the mechanisms of sensorineural hearing loss using metabolomic analysis. View Full-Text
Keywords: cochlear implant; metabolomics; perilymph; duration of hearing loss cochlear implant; metabolomics; perilymph; duration of hearing loss
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Trinh, T.-T.; Blasco, H.; Emond, P.; Andres, C.; Lefevre, A.; Lescanne, E.; Bakhos, D. Relationship between Metabolomics Profile of Perilymph in Cochlear-Implanted Patients and Duration of Hearing Loss. Metabolites 2019, 9, 262.

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