Metabolic Profiling of Plasma from Benign and Malignant Pulmonary Nodules Patients Using Mass Spectrometry-Based Metabolomics
Abstract
:1. Introduction
2. Results and Discussion
2.1. Patient Characteristics
Healthy controls | Pulmonary benign | Pulmonary malignant | |
---|---|---|---|
nodules | nodules | ||
No. of subjects | 20 | 15 | 17 |
Gender | Male | Male | Male |
Age range (median) | 29–50 (38) | 33–79 (59) | 49–67(60) |
Smoking habits | Smokers 6 | Smokers 9 | Smokers 17 |
Non-smokers 14 | Non-smokers 6 | Non-smokers 0 | |
Nodule size (average) | 1.9 cm | 2.2 cm | |
Histological type | Squamous cell carcinoma | ||
Cancer stage | Ⅰ |
2.1. GC/MS Analysis
2.2. LC/MS Analysis
2.3. Discussion
3. Experimental Section
3.1. Clinical Populations
3.2. Plasma sample Collection
3.3. Chemicals
3.4. Sample Pretreatment
3.5. GC/MS Analysis
3.6. LC/MS Analysis
3.7. Data Preprocessing
3.8. Chemometrics Data Analysis
3.9. Marker Metabolites Selection and Identification
4. Conclusions
Acknowledgments
Conflict of Interest
References
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Gao, L.; Wen, Z.; Wu, C.; Wen, T.; Ong, C.N. Metabolic Profiling of Plasma from Benign and Malignant Pulmonary Nodules Patients Using Mass Spectrometry-Based Metabolomics. Metabolites 2013, 3, 539-551. https://doi.org/10.3390/metabo3030539
Gao L, Wen Z, Wu C, Wen T, Ong CN. Metabolic Profiling of Plasma from Benign and Malignant Pulmonary Nodules Patients Using Mass Spectrometry-Based Metabolomics. Metabolites. 2013; 3(3):539-551. https://doi.org/10.3390/metabo3030539
Chicago/Turabian StyleGao, Liang, Zongmei Wen, Chunyan Wu, Tao Wen, and Choon Nam Ong. 2013. "Metabolic Profiling of Plasma from Benign and Malignant Pulmonary Nodules Patients Using Mass Spectrometry-Based Metabolomics" Metabolites 3, no. 3: 539-551. https://doi.org/10.3390/metabo3030539