Mass Spectrometry Based Lipidomics: An Overview of Technological Platforms
Abstract
:1. Introduction
2. Direct Infusion
3. LC-MS
3.1. Low Resolution Mass Spectrometry
3.2. High Resolution Mass Spectrometry
3.3. Digging Deeper into Structural Details
4. MALDI-TOF
5. Data Analysis
Data type | MS vendor restrictions | Recommended instruments | Identification | Quanti- tation | Availability | |
---|---|---|---|---|---|---|
Lipid Inspector | shotgun | all possible | triple quadrupole, quadrupole-TOF | MPIS | Yes | upon request |
Lipid View | shotgun | only AB Sciex | triple quadrupole, quadrupole-TOF | MPIS | Yes | purchase |
LipidXplorer | shotgun | all possible | orbitrap, quadrupole-TOF | data dependent acquisition | Yes | open source |
m/z Mine 2 | LC-MS | all possible | quadrupole-TOF | HiRes MS full scan, retention time | Yes | open source |
Lipid Data Analyzer | LC-MS | all possible | FT-ICR-MS | HiRes MS full scan, retention time | Yes | open source |
6. Conclusions
Acknowledgments
Conflict of Interest
References and Notes
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Köfeler, H.C.; Fauland, A.; Rechberger, G.N.; Trötzmüller, M. Mass Spectrometry Based Lipidomics: An Overview of Technological Platforms. Metabolites 2012, 2, 19-38. https://doi.org/10.3390/metabo2010019
Köfeler HC, Fauland A, Rechberger GN, Trötzmüller M. Mass Spectrometry Based Lipidomics: An Overview of Technological Platforms. Metabolites. 2012; 2(1):19-38. https://doi.org/10.3390/metabo2010019
Chicago/Turabian StyleKöfeler, Harald C., Alexander Fauland, Gerald N. Rechberger, and Martin Trötzmüller. 2012. "Mass Spectrometry Based Lipidomics: An Overview of Technological Platforms" Metabolites 2, no. 1: 19-38. https://doi.org/10.3390/metabo2010019