Changes in a Protein Profile Can Account for the Altered Phenotype of the Yeast Saccharomyces cerevisiae Mutant Lacking the Copper-Zinc Superoxide Dismutase
Abstract
1. Introduction
2. Materials and Methods
2.1. Yeast Strains and Growth Conditions
2.2. Protein Extraction
2.3. Two-Dimensional Gel Electrophoresis (2-DE)
2.4. Image Analysis and Statistics
2.5. Mass Spectrometry
2.6. Protein Identification
2.7. Western Blot
2.8. RNA Samples
2.9. Real-Time PCR
3. Results
3.1. Comparative Proteome Analysis
3.2. Identification of Proteins
3.3. Functional Classes of the Identified Proteins
3.4. Decrease of Methionine Synthase Expression Causes Methionine Auxotrophy in the Δsod1 Mutant
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Spot | Accession Number (ORF) | Protein Name | Description | Molecular Mass [kDa] | pI b | MOWSE Score | Number of Peptides Matched | Sequence Coverage [%] | emPAI | |
---|---|---|---|---|---|---|---|---|---|---|
Calculated a | Theoretical b | |||||||||
1. | YOR133W YDR385W | EFT1 EFT2 p | Elongation factor 2 Elongation factor 2 | 93.69 93.69 | 93.28 93.28 | 6.23 6.23 | 3559 3559 | 72 72 | 39 39 | 5.85 5.85 |
2. | YER091C | MET6 | Cobalamin-independent methionine synthase | 85.98 | 85.85 | 6.41 | 3703 | 64 | 35 | 5.02 |
3. | YMR120C YLR028C | ADE17 ADE16 p | Enzyme of ‘de novo’ purine biosynthesis Enzyme of ‘de novo’ purine biosynthesis | 65.56 65.64 | 65.26 65.28 | 6.52 6.52 | 2280 1075 | 41 18 | 26 14 | 4.02 1.19 |
4. | YLR044C | PDC1 | Major of three pyruvate decarboxylase isoenzymes | 61.68 | 61.49 | 6.11 | 2856 | 54 | 19 | 4.54 |
5. | YBR248C | HIS7 | Imidazole glycerol phosphate synthase | 61.54 | 61.05 | 5.15 | 1219 | 22 | 18 | 1.83 |
6. | YPR080W YBR118W | TEF1 TEF2 p | Translational elongation factor 1 alpha Translational elongation factor 1 alpha | 50.40 50.40 | 50.04 50.04 | 9.58 9.58 | 684 684 | 16 16 | 10 10 | 1.01 1.01 |
7. | YHR174W | ENO2 | Enolase II, a phosphopyruvate hydratase | 46.94 | 46.91 | 5.85 | 9716 | 150 | 35 | 43.84 |
8. | YHR068W | DYS1 | Deoxyhypusine synthase | 43.26 | 42.88 | 5.49 | 643 | 11 | 9 | 1.09 |
9. | YCL018W | LEU2 | Beta-isopropylmalate dehydrogenase | 39.04 | 38.95 | 5.48 | 872 | 16 | 15 | 2.67 |
10. | YOL086C | ADH1 | Alcohol dehydrogenase I | 37.28 | 36.84 | 6.67 | 4286 | 89 | 20 | 9.83 |
11. | YKL060C | FBA1 | Fructose 1,6-bisphosphate aldolase | 39.88 | 39.61 | 5.59 | 1043 | 18 | 11 | 2.05 |
12. | YJR105W | ADO1 | Adenosine kinase | 36.52 | 36.36 | 4.77 | 1047 | 15 | 10 | 1.84 |
13. | YGR192C | TDH3 | Glyceraldehyde-3-phosphate dehydrogenase | 35.84 | 35.74 | 6.96 | 1978 | 39 | 21 | 7.35 |
14. | YKL152C | GPM1 | Tetrameric phosphoglycerate mutase | 27.59 | 27.61 | 9.34 | 706 | 13 | 10 | 2.92 |
15. | YML028W | TSA1 | Thioredoxin peroxidase | 21.69 | 21.58 | 4.77 | 957 | 20 | 9 | 3.88 |
16. | YDR454C | GUK1 | Guanylate kinase | 20.68 | 20.64 | 7.34 | 789 | 15 | 7 | 2.88 |
17. | YLR109W | AHP1 | Thiol-specific peroxiredoxin | 19.27 | 19.11 | 4.78 | 831 | 13 | 9 | 3.99 |
18. | YJR104C | SOD1 | Cytosolic copper-zinc superoxide dismutase | 15.96 | 15.85 | 5.91 | 963 | 16 | 10 | 9.06 |
19. | YDR382W | RPP2B | Ribosomal protein P2 beta | 11.04 | 11.03 | 3.80 | 793 | 11 | 3 | 1.27 |
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Kwolek-Mirek, M.; Dubicka-Lisowska, A.; Bednarska, S.; Zadrag-Tecza, R.; Kaszycki, P. Changes in a Protein Profile Can Account for the Altered Phenotype of the Yeast Saccharomyces cerevisiae Mutant Lacking the Copper-Zinc Superoxide Dismutase. Metabolites 2023, 13, 459. https://doi.org/10.3390/metabo13030459
Kwolek-Mirek M, Dubicka-Lisowska A, Bednarska S, Zadrag-Tecza R, Kaszycki P. Changes in a Protein Profile Can Account for the Altered Phenotype of the Yeast Saccharomyces cerevisiae Mutant Lacking the Copper-Zinc Superoxide Dismutase. Metabolites. 2023; 13(3):459. https://doi.org/10.3390/metabo13030459
Chicago/Turabian StyleKwolek-Mirek, Magdalena, Aleksandra Dubicka-Lisowska, Sabina Bednarska, Renata Zadrag-Tecza, and Pawel Kaszycki. 2023. "Changes in a Protein Profile Can Account for the Altered Phenotype of the Yeast Saccharomyces cerevisiae Mutant Lacking the Copper-Zinc Superoxide Dismutase" Metabolites 13, no. 3: 459. https://doi.org/10.3390/metabo13030459
APA StyleKwolek-Mirek, M., Dubicka-Lisowska, A., Bednarska, S., Zadrag-Tecza, R., & Kaszycki, P. (2023). Changes in a Protein Profile Can Account for the Altered Phenotype of the Yeast Saccharomyces cerevisiae Mutant Lacking the Copper-Zinc Superoxide Dismutase. Metabolites, 13(3), 459. https://doi.org/10.3390/metabo13030459