Integrated Metabolomics and Transcriptomics Reveal Metabolic Patterns in Retina of STZ-Induced Diabetic Retinopathy Mouse Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Optical Coherence Tomography (OCT)
2.3. Isolation of Retinas
2.4. Non-Targeted Metabolomics by UHPLC-MS/MS
2.5. Non-Targeted Metabolomics by GC-MS
2.6. RNA Sequencing Analysis
2.7. Genome-Wide Association Study (GWAS) Analysis
2.8. Statistics Analysis
3. Results
3.1. The Thickness of Inner Retina was Altered in STZ-Induced Mice
3.2. Metabolomics Displays Signatures of Metabolism Dysregulation in Diabetic Mice Retinas
3.3. RNA-Sequencing Analysis Reveals the Alterations of Metabolic-Related Genes in the Diabetic Retinas of Mice
3.4. Integrated Transcriptome–Metabolome Analysis Reveals the Characteristics of Metabolic Regulators in Diabetic Mouse Retina
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Metabolic Pathway | Differentially Expressed Gene |
---|---|
Warburg Effect | Hk2, Hras, Kit, Met, Nras, Ntrk1, Pgam1, Slc7a5 *, Trp53 |
Valine, leucine and isoleucine degradation | Acaa2, Acsf3, Aldh1b1, Ivd |
Oxidative phosphorylation | Atp6v0c, Atp6v1f, Cox6b1, Cycs, Ndufb2, Ndufb3, Ndufv3, Ppa1, Tcirg1, Uqcr10 *, Uqcrb |
Arginine and proline metabolism | Aldh4a1, Azin2, Ckmt1, Lap3, Maob, Nos2, Smox |
Alanine, aspartate and glutamate metabolism | Asns |
Cysteine and methionine metabolism | Bhmt, Cbs, Mat1a, Mri1, Mtap* |
Tryptophan metabolism | Tph2 |
Pentose phosphate pathway | Rgn |
Pyrimidine metabolism | Cda, Cmpk2, Ctps, Ctps2, Dhodh, Entpd3 |
Starch and sucrose metabolism | Pygm |
Glycerolipid metabolism | Agpat1 *, Agpat4, Dgkb*, Lipg, Lpin3, Mboat1 *, Plpp2, Pnliprp2 |
Glycolysis | Aldh3a1, Pck1, Pgk1 |
Inositol Phosphate Metabolism | Ipmk, Pip4k2a |
Sphingolipid metabolism | 9130409I23Rik, Asah2, Hexa, Neu3, Smpd1 |
Steroid biosynthesis | Lss, Nsdhl |
Retinol metabolism | Aldh1a3, Aldh1a7, Cyp26b1, Cyp3a25, Ugt1a6a |
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Wang, R.; Jian, Q.; Hu, G.; Du, R.; Xu, X.; Zhang, F. Integrated Metabolomics and Transcriptomics Reveal Metabolic Patterns in Retina of STZ-Induced Diabetic Retinopathy Mouse Model. Metabolites 2022, 12, 1245. https://doi.org/10.3390/metabo12121245
Wang R, Jian Q, Hu G, Du R, Xu X, Zhang F. Integrated Metabolomics and Transcriptomics Reveal Metabolic Patterns in Retina of STZ-Induced Diabetic Retinopathy Mouse Model. Metabolites. 2022; 12(12):1245. https://doi.org/10.3390/metabo12121245
Chicago/Turabian StyleWang, Ruonan, Qizhi Jian, Guangyi Hu, Rui Du, Xun Xu, and Fang Zhang. 2022. "Integrated Metabolomics and Transcriptomics Reveal Metabolic Patterns in Retina of STZ-Induced Diabetic Retinopathy Mouse Model" Metabolites 12, no. 12: 1245. https://doi.org/10.3390/metabo12121245