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Article

First Genome-Scale Metabolic Model of Dolosigranulum pigrum Confirms Multiple Auxotrophies

by 1,2,3, 1 and 1,2,3,4,*
1
Computational Systems Biology of Infections and Antimicrobial-Resistant Pathogens, Institute for Bioinformatics and Medical Informatics (IBMI), University of Tübingen, 72076 Tübingen, Germany
2
Department of Computer Science, University of Tübingen, 72076 Tübingen, Germany
3
Cluster of Excellence ‘Controlling Microbes to Fight Infections’, University of Tübingen, 72076 Tübingen, Germany
4
German Center for Infection Research (DZIF), Partner site Tübingen, 72076 Tübingen, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Brian Drew
Metabolites 2021, 11(4), 232; https://doi.org/10.3390/metabo11040232
Received: 18 February 2021 / Revised: 21 March 2021 / Accepted: 6 April 2021 / Published: 9 April 2021
Dolosigranulum pigrum is a quite recently discovered Gram-positive coccus. It has gained increasing attention due to its negative correlation with Staphylococcus aureus, which is one of the most successful modern pathogens causing severe infections with tremendous morbidity and mortality due to its multiple resistances. As the possible mechanisms behind its inhibition of S. aureus remain unclear, a genome-scale metabolic model (GEM) is of enormous interest and high importance to better study its role in this fight. This article presents the first GEM of D. pigrum, which was curated using automated reconstruction tools and extensive manual curation steps to yield a high-quality GEM. It was evaluated and validated using all currently available experimental data of D. pigrum. With this model, already predicted auxotrophies and biosynthetic pathways could be verified. The model was used to define a minimal medium for further laboratory experiments and to predict various carbon sources’ growth capacities. This model will pave the way to better understand D. pigrum’s role in the fight against S. aureus. View Full-Text
Keywords: Dolosigranulum pigrum; genome-scale metabolic model; Staphylococcus aureus; interaction; auxotrophy; nose microbiome Dolosigranulum pigrum; genome-scale metabolic model; Staphylococcus aureus; interaction; auxotrophy; nose microbiome
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MDPI and ACS Style

Renz, A.; Widerspick, L.; Dräger, A. First Genome-Scale Metabolic Model of Dolosigranulum pigrum Confirms Multiple Auxotrophies. Metabolites 2021, 11, 232. https://doi.org/10.3390/metabo11040232

AMA Style

Renz A, Widerspick L, Dräger A. First Genome-Scale Metabolic Model of Dolosigranulum pigrum Confirms Multiple Auxotrophies. Metabolites. 2021; 11(4):232. https://doi.org/10.3390/metabo11040232

Chicago/Turabian Style

Renz, Alina, Lina Widerspick, and Andreas Dräger. 2021. "First Genome-Scale Metabolic Model of Dolosigranulum pigrum Confirms Multiple Auxotrophies" Metabolites 11, no. 4: 232. https://doi.org/10.3390/metabo11040232

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