l-Theanine Protects Bladder Function by Suppressing Chronic Sympathetic Hyperactivity in Spontaneously Hypertensive Rat
Abstract
:1. Introduction
2. Results
2.1. Voiding Behaviors in SHRs after 6 Weeks
2.2. Cystometric Parameters in Conscious Rats
2.3. Assessment of Bladder Contractile Strength
2.4. Expression of Oxidative Stress Markers in the Bladder
2.5. Histological Changes in the Bladder Muscle Layer
3. Discussion
4. Materials and Methods
4.1. Animals and Experimental Design
4.2. Metabolic Cage Experiments
4.3. Conscious Free-Moving Cystometry
4.4. Organ Bath Study
4.5. Detection of Oxidative Stress Markers
4.6. Histological Examination
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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SHR-Water | SHR-Theanine | p Value | |||||
---|---|---|---|---|---|---|---|
Fluid intake (mL/day) | 33.9 | ± | 2.8 | 33.8 | ± | 1.7 | 1.000 |
Body weight (g) | 353.0 | ± | 12.3 | 354.0 | ± | 11.3 | 0.912 |
Bladder weight (g) | 0.19 | ± | 0.03 | 0.22 | ± | 0.04 | 0.515 |
HR (beats/min) | 412.3 | ± | 18.5 | 391.9 | ± | 20.7 | 0.606 |
SBP (mmHg) | 204.0 | ± | 5.8 | 188.8 | ± | 3.8 | 0.074 |
MBP (mmHg) | 173.3 | ± | 4.3 | 159.9 | ± | 3.3 | 0.046 * |
DBP (mmHg) | 159.3 | ± | 3.9 | 147.9 | ± | 4.1 | 0.074 |
SHR-Water | SHR-Theanine | p Value | |||||
---|---|---|---|---|---|---|---|
Urine volume (g/24-h) | 14.4 | ± | 3.3 | 12.9 | ± | 3.2 | 0.393 |
Micturition frequency (count/24-h) | 22.3 | ± | 5.3 | 16.3 | ± | 4.0 | 0.004 * |
Daytime frequency (count/12-h) | 5.0 | ± | 1.6 | 5.1 | ± | 1.2 | 0.796 |
Nighttime frequency (count/12-h) | 16.3 | ± | 4.3 | 11.2 | ± | 3.6 | 0.007 * |
Single voided volume (g) | 0.67 | ± | 0.19 | 0.81 | ± | 0.15 | 0.043 * |
SHR-Water | SHR-Theanine | p Value | |||||
---|---|---|---|---|---|---|---|
Intercontractile interval (min) | 5.6 | ± | 8.4 | 7.8 | ± | 1.7 | 0.002 * |
Baseline pressure (cmH2O) | 9.59 | ± | 5.56 | 8.93 | ± | 4.83 | 0.739 |
Voided volume (g) | 0.96 | ± | 0.18 | 1.34 | ± | 0.35 | 0.029 * |
Residual urine volume (mL) | 0.16 | ± | 0.08 | 0.22 | ± | 0.13 | 0.247 |
Maximum pressure (cmH2O) | 47.1 | ± | 16.9 | 70.4 | ± | 33.1 | 0.123 |
Bladder compliance (g/cmH2O) | 0.12 | ± | 0.79 | 0.11 | ± | 0.03 | 0.393 |
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Matsuoka, K.; Akaihata, H.; Hata, J.; Tanji, R.; Honda-Takinami, R.; Onagi, A.; Hoshi, S.; Koguchi, T.; Sato, Y.; Kataoka, M.; et al. l-Theanine Protects Bladder Function by Suppressing Chronic Sympathetic Hyperactivity in Spontaneously Hypertensive Rat. Metabolites 2021, 11, 778. https://doi.org/10.3390/metabo11110778
Matsuoka K, Akaihata H, Hata J, Tanji R, Honda-Takinami R, Onagi A, Hoshi S, Koguchi T, Sato Y, Kataoka M, et al. l-Theanine Protects Bladder Function by Suppressing Chronic Sympathetic Hyperactivity in Spontaneously Hypertensive Rat. Metabolites. 2021; 11(11):778. https://doi.org/10.3390/metabo11110778
Chicago/Turabian StyleMatsuoka, Kanako, Hidenori Akaihata, Junya Hata, Ryo Tanji, Ruriko Honda-Takinami, Akifumi Onagi, Seiji Hoshi, Tomoyuki Koguchi, Yuichi Sato, Masao Kataoka, and et al. 2021. "l-Theanine Protects Bladder Function by Suppressing Chronic Sympathetic Hyperactivity in Spontaneously Hypertensive Rat" Metabolites 11, no. 11: 778. https://doi.org/10.3390/metabo11110778