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Open AccessArticle

Application of Carbon-13 Isotopomer Analysis to Assess Perinatal Myocardial Glucose Metabolism in Sheep

1
Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32603, USA
2
Department of Pharmacodynamics, University of Florida, Gainesville, FL 32610, USA
3
Department of Physiology and Functional Genomics, University of Florida, Gainesville, FL 32603, USA
4
Donald H Barron Reproductive and Perinatal Biology Research Program, University of Florida, Gainesville, FL 32603, USA
*
Author to whom correspondence should be addressed.
Metabolites 2021, 11(1), 33; https://doi.org/10.3390/metabo11010033
Received: 23 November 2020 / Revised: 16 December 2020 / Accepted: 1 January 2021 / Published: 5 January 2021
(This article belongs to the Special Issue Stable Isotope Guided Metabolomics)
Ovine models of pregnancy have been used extensively to study maternal–fetal interactions and have provided considerable insight into nutrient transfer to the fetus. Ovine models have also been utilized to study congenital heart diseases. In this work, we demonstrate a comprehensive assessment of heart function and metabolism using a perinatal model of heart function with the addition of a [U-13C]glucose as tracer to study central energy metabolism. Using nuclear magnetic resonance spectroscopy, and metabolic modelling, we estimate myocardial citric acid cycle turnover (normalized for oxygen consumption), substrate selection, and anaplerotic fluxes. This methodology can be applied to studying acute and chronic effects of hormonal signaling in future studies. View Full-Text
Keywords: isotopomer analysis; stable isotope tracer methodology; fluxomics; cardiac metabolism; fetal heart metabolism isotopomer analysis; stable isotope tracer methodology; fluxomics; cardiac metabolism; fetal heart metabolism
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MDPI and ACS Style

Ragavan, M.; Li, M.; Giacalone, A.G.; Wood, C.E.; Keller-Wood, M.; Merritt, M.E. Application of Carbon-13 Isotopomer Analysis to Assess Perinatal Myocardial Glucose Metabolism in Sheep. Metabolites 2021, 11, 33. https://doi.org/10.3390/metabo11010033

AMA Style

Ragavan M, Li M, Giacalone AG, Wood CE, Keller-Wood M, Merritt ME. Application of Carbon-13 Isotopomer Analysis to Assess Perinatal Myocardial Glucose Metabolism in Sheep. Metabolites. 2021; 11(1):33. https://doi.org/10.3390/metabo11010033

Chicago/Turabian Style

Ragavan, Mukundan; Li, Mengchen; Giacalone, Anthony G.; Wood, Charles E.; Keller-Wood, Maureen; Merritt, Matthew E. 2021. "Application of Carbon-13 Isotopomer Analysis to Assess Perinatal Myocardial Glucose Metabolism in Sheep" Metabolites 11, no. 1: 33. https://doi.org/10.3390/metabo11010033

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