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Article

Effects of Water Deficit and Heat Stress on Nicotiana langsdorffii Metabolomic Pattern Modified by Insertion of rolD Gene from Agrobacterium rhizogenes

Department of Environmental Sciences, Informatics and Statistics, Ca’Foscari University of Venice, Via Torino 155, Mestre, 30173 Venezia, Italy
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Metabolites 2020, 10(8), 310; https://doi.org/10.3390/metabo10080310
Received: 24 April 2020 / Revised: 23 July 2020 / Accepted: 24 July 2020 / Published: 29 July 2020
(This article belongs to the Special Issue Phenolic Compounds and Metabolome)
Abiotic stresses are major factors that negatively affect plant growth and productivity. Plants have developed complex strategies to ensure their survival and reproduction under adverse conditions, activating mechanisms that involve changes at different metabolic levels. In order to select stress-resistant species, research has focused on molecular studies and genetic engineering, showing promising results. In this work, the insertion of the rolD gene from Agrobacterium rhizogenes into Nicotiana langsdorffii plants is investigated, in order to assess the potential of this genetic modification towards mitigating water and heat stresses. Different approaches were combined: a high-throughput metabolomics and ionomics study was performed, together with the determination of important plant phytohormones. The aim was to identify the influence of abiotic stresses on plants and to highlight the effects of the rolD genetic modification on plant stress response. The most relevant compounds for each kind of stress were identified, belonging mainly to the classes of lipids, acyl sugars, glycosides, and amino acid derivatives. Water stress (WS) determined a decrease of elements and secondary metabolites, while amino acids and their derivatives increased, proving to be key molecules in this type of stress. RolD plants exposed to high temperature stress (HS) presented higher dry weight levels than controls, as well as increased amounts of K and adenosine and lower levels of damage-associated metabolites, suggesting the increased resistance of rolD-modified plants toward HS. View Full-Text
Keywords: abiotic stresses; ionomics; metabolomics; Nicotiana langsdorffii; plant metabolome; rolD abiotic stresses; ionomics; metabolomics; Nicotiana langsdorffii; plant metabolome; rolD
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MDPI and ACS Style

Scalabrin, E.; Radaelli, M.; Capodaglio, G. Effects of Water Deficit and Heat Stress on Nicotiana langsdorffii Metabolomic Pattern Modified by Insertion of rolD Gene from Agrobacterium rhizogenes. Metabolites 2020, 10, 310. https://doi.org/10.3390/metabo10080310

AMA Style

Scalabrin E, Radaelli M, Capodaglio G. Effects of Water Deficit and Heat Stress on Nicotiana langsdorffii Metabolomic Pattern Modified by Insertion of rolD Gene from Agrobacterium rhizogenes. Metabolites. 2020; 10(8):310. https://doi.org/10.3390/metabo10080310

Chicago/Turabian Style

Scalabrin, Elisa, Marta Radaelli, and Gabriele Capodaglio. 2020. "Effects of Water Deficit and Heat Stress on Nicotiana langsdorffii Metabolomic Pattern Modified by Insertion of rolD Gene from Agrobacterium rhizogenes" Metabolites 10, no. 8: 310. https://doi.org/10.3390/metabo10080310

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