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Article

Application of Multiblock Analysis on Small Metabolomic Multi-Tissue Dataset

by 1,†, 1,† and 1,2,*
1
Computational Life Science Cluster (CLiC), Department of Chemistry, Umeå University, 90187 Umeå, Sweden
2
Corporate Research, Sartorius, 90187 Umeå, Sweden
*
Author to whom correspondence should be addressed.
These authors have contributed equally to this work.
Metabolites 2020, 10(7), 295; https://doi.org/10.3390/metabo10070295
Received: 27 May 2020 / Revised: 14 July 2020 / Accepted: 15 July 2020 / Published: 17 July 2020
(This article belongs to the Section Bioinformatics and Data Analysis)
Data integration has been proven to provide valuable information. The information extracted using data integration in the form of multiblock analysis can pinpoint both common and unique trends in the different blocks. When working with small multiblock datasets the number of possible integration methods is drastically reduced. To investigate the application of multiblock analysis in cases where one has a few number of samples and a lack of statistical power, we studied a small metabolomic multiblock dataset containing six blocks (i.e., tissue types), only including common metabolites. We used a single model multiblock analysis method called the joint and unique multiblock analysis (JUMBA) and compared it to a commonly used method, concatenated principal component analysis (PCA). These methods were used to detect trends in the dataset and identify underlying factors responsible for metabolic variations. Using JUMBA, we were able to interpret the extracted components and link them to relevant biological properties. JUMBA shows how the observations are related to one another, the stability of these relationships, and to what extent each of the blocks contribute to the components. These results indicate that multiblock methods can be useful even with a small number of samples. View Full-Text
Keywords: data integration; metabolomics; multi-tissue; multiblock; joint and unique multiblock analysis (JUMBA); OnPLS; multiblock orthogonal component analysis (MOCA) data integration; metabolomics; multi-tissue; multiblock; joint and unique multiblock analysis (JUMBA); OnPLS; multiblock orthogonal component analysis (MOCA)
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MDPI and ACS Style

Torell, F.; Skotare, T.; Trygg, J. Application of Multiblock Analysis on Small Metabolomic Multi-Tissue Dataset. Metabolites 2020, 10, 295. https://doi.org/10.3390/metabo10070295

AMA Style

Torell F, Skotare T, Trygg J. Application of Multiblock Analysis on Small Metabolomic Multi-Tissue Dataset. Metabolites. 2020; 10(7):295. https://doi.org/10.3390/metabo10070295

Chicago/Turabian Style

Torell, Frida, Tomas Skotare, and Johan Trygg. 2020. "Application of Multiblock Analysis on Small Metabolomic Multi-Tissue Dataset" Metabolites 10, no. 7: 295. https://doi.org/10.3390/metabo10070295

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