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Article

Sphingolipid Profiling Reveals Different Extent of Ceramide Accumulation in Bovine Retroperitoneal and Subcutaneous Adipose Tissues

1
Department of Infectious Diseases and Public Health, City University of Hong Kong, Hong Kong
2
Institute of Animal Science, University of Hohenheim, 70599 Stuttgart, Germany
3
Educational and Research Centre for Animal Husbandry, Hofgut Neumuehle, 67728 Muenchweiler a.d. Alsenz, Germany
4
Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, 38116 Braunschweig, Germany
*
Author to whom correspondence should be addressed.
Metabolites 2020, 10(11), 473; https://doi.org/10.3390/metabo10110473
Received: 8 October 2020 / Revised: 5 November 2020 / Accepted: 17 November 2020 / Published: 19 November 2020
(This article belongs to the Section Lipid Metabolism)
Sphingolipids are bioactive lipids that can modulate insulin sensitivity, cellular differentiation, and apoptosis in a tissue-specific manner. However, their comparative profiles in bovine retroperitoneal (RPAT) and subcutaneous adipose tissue (SCAT) are currently unknown. We aimed to characterize the sphingolipid profiles using a targeted lipidomics approach and to assess whether potentially related sphingolipid pathways are different between SCAT and RPAT. Holstein bulls (n = 6) were slaughtered, and SCAT and RPAT samples were collected for sphingolipid profiling. A total of 70 sphingolipid species were detected and quantified by UPLC-MS/MS in multiple reaction monitoring (MRM) mode, including ceramide (Cer), dihydroceramide (DHCer), sphingomyelin (SM), dihydrosphingomyelin (DHSM), ceramide-1-phosphate (C1P), sphingosine-1-phosphate (S1P), galactosylceramide (GalCer), glucosylceramide (GluCer), lactosylceramide (LacCer), sphinganine (DHSph), and sphingosine (Sph). Our results showed that sphingolipids of the de novo synthesis pathway, such as DHSph, DHCer, and Cer, were more concentrated in RPAT than in SCAT. Sphingolipids of the salvage pathway and the sphingomyelinase pathway, such as Sph, S1P, C1P, glycosphingolipid, and SM, were more concentrated in SCAT. Our results indicate that RPAT had a greater extent of ceramide accumulation, thereby increasing the concentration of further sphingolipid intermediates in the de novo synthesis pathway. This distinctive sphingolipid distribution pattern in RPAT and SCAT can potentially explain the tissue-specific activity in insulin sensitivity, proinflammation, and oxidative stress in RPAT and SCAT. View Full-Text
Keywords: sphingolipids; ceramides; adipose tissue biology; retroperitoneal adipose tissue; subcutaneous adipose tissue; bovine sphingolipids; ceramides; adipose tissue biology; retroperitoneal adipose tissue; subcutaneous adipose tissue; bovine
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MDPI and ACS Style

Leung, Y.H.; Christiane Bäßler, S.; Koch, C.; Scheu, T.; Meyer, U.; Dänicke, S.; Huber, K.; Kenéz, Á. Sphingolipid Profiling Reveals Different Extent of Ceramide Accumulation in Bovine Retroperitoneal and Subcutaneous Adipose Tissues. Metabolites 2020, 10, 473. https://doi.org/10.3390/metabo10110473

AMA Style

Leung YH, Christiane Bäßler S, Koch C, Scheu T, Meyer U, Dänicke S, Huber K, Kenéz Á. Sphingolipid Profiling Reveals Different Extent of Ceramide Accumulation in Bovine Retroperitoneal and Subcutaneous Adipose Tissues. Metabolites. 2020; 10(11):473. https://doi.org/10.3390/metabo10110473

Chicago/Turabian Style

Leung, Yue H., Sonja Christiane Bäßler, Christian Koch, Theresa Scheu, Ulrich Meyer, Sven Dänicke, Korinna Huber, and Ákos Kenéz. 2020. "Sphingolipid Profiling Reveals Different Extent of Ceramide Accumulation in Bovine Retroperitoneal and Subcutaneous Adipose Tissues" Metabolites 10, no. 11: 473. https://doi.org/10.3390/metabo10110473

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