Multi-Targeted Metabolic Profiling of Carotenoids, Phenolic Compounds and Primary Metabolites in Goji (Lycium spp.) Berry and Tomato (Solanum lycopersicum) Reveals Inter and Intra Genus Biomarkers
Abstract
:1. Introduction
2. Results and Discussion
2.1. Carotenoid Profiling Optimization and Validation
2.1.1. Extraction
2.1.2. UPLC–MS
2.1.3. Quantification
2.2. Phenolic Compound Profiling Optimization and Validation
2.2.1. UPLC–MS
2.2.2. Quantification
2.3. Primary Metabolite Profiling Optimization and Validation
2.4. Application to the Comparison of Several Solanaceae Species
3. Materials and Methods
3.1. Plant Materials
3.2. Metabolite Extraction
3.2.1. Carotenoids
3.2.2. Phenolic Compounds
3.2.3. Primary Metabolites
3.3. Metabolic Profiling by UPLC–DAD–ESI–TQ and GC–EI–TOF
3.3.1. UPLC–DAD–ESI–TQ Analysis
3.3.2. GC–EI–TOF Analysis
3.3.3. Data Processing and Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Dumont, D.; Danielato, G.; Chastellier, A.; Hibrand Saint Oyant, L.; Fanciullino, A.-L.; Lugan, R. Multi-Targeted Metabolic Profiling of Carotenoids, Phenolic Compounds and Primary Metabolites in Goji (Lycium spp.) Berry and Tomato (Solanum lycopersicum) Reveals Inter and Intra Genus Biomarkers. Metabolites 2020, 10, 422. https://doi.org/10.3390/metabo10100422
Dumont D, Danielato G, Chastellier A, Hibrand Saint Oyant L, Fanciullino A-L, Lugan R. Multi-Targeted Metabolic Profiling of Carotenoids, Phenolic Compounds and Primary Metabolites in Goji (Lycium spp.) Berry and Tomato (Solanum lycopersicum) Reveals Inter and Intra Genus Biomarkers. Metabolites. 2020; 10(10):422. https://doi.org/10.3390/metabo10100422
Chicago/Turabian StyleDumont, Doriane, Giorgia Danielato, Annie Chastellier, Laurence Hibrand Saint Oyant, Anne-Laure Fanciullino, and Raphaël Lugan. 2020. "Multi-Targeted Metabolic Profiling of Carotenoids, Phenolic Compounds and Primary Metabolites in Goji (Lycium spp.) Berry and Tomato (Solanum lycopersicum) Reveals Inter and Intra Genus Biomarkers" Metabolites 10, no. 10: 422. https://doi.org/10.3390/metabo10100422