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Metabolites from Microbes Isolated from the Skin of the Panamanian Rocket Frog Colostethus panamansis (Anura: Dendrobatidae)

1
Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Clayton, Panama 0843-01103, Panama
2
Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur 522510, India
3
Smithsonian Tropical Research Institute, Balboa, Ancon, Panama 0843-03092, Panama
4
Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA 92093, USA
*
Author to whom correspondence should be addressed.
Metabolites 2020, 10(10), 406; https://doi.org/10.3390/metabo10100406
Received: 14 September 2020 / Revised: 28 September 2020 / Accepted: 9 October 2020 / Published: 13 October 2020
(This article belongs to the Special Issue Microbiome and Metabolome)
The Panamanian rocket frog Colostethus panamansis (family Dendrobatidae) has been affected by chytridiomycosis, a deadly disease caused by the fungus Batrachochytrium dendrobatidis (Bd). While there are still uninfected frogs, we set out to isolate microbes from anatomically distinct regions in an effort to create a cultivable resource within Panama for potential drug/agricultural/ecological applications that perhaps could also be used as part of a strategy to protect frogs from infections. To understand if there are specific anatomies that should be explored in future applications of this resource, we mapped skin-associated bacteria of C. panamansis and their metabolite production potential by mass spectrometry on a 3D model. Our results indicate that five bacterial families (Enterobacteriaceae, Comamonadaceae, Aeromonadaceae, Staphylococcaceae and Pseudomonadaceae) dominate the cultivable microbes from the skin of C. panamansis. The combination of microbial classification and molecular analysis in relation to the anti-Bd inhibitory databases reveals the resource has future potential for amphibian conservation. View Full-Text
Keywords: 3D molecular cartography; Colostethus panamansis; Dendrobatidae; skin-associated bacteria; 16S rRNA sequencing; feature-based molecular networking 3D molecular cartography; Colostethus panamansis; Dendrobatidae; skin-associated bacteria; 16S rRNA sequencing; feature-based molecular networking
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MDPI and ACS Style

Martin H., C.; Ibáñez, R.; Nothias, L.-F.; Caraballo-Rodríguez, A.M.; Dorrestein, P.C.; Gutiérrez, M. Metabolites from Microbes Isolated from the Skin of the Panamanian Rocket Frog Colostethus panamansis (Anura: Dendrobatidae). Metabolites 2020, 10, 406. https://doi.org/10.3390/metabo10100406

AMA Style

Martin H. C, Ibáñez R, Nothias L-F, Caraballo-Rodríguez AM, Dorrestein PC, Gutiérrez M. Metabolites from Microbes Isolated from the Skin of the Panamanian Rocket Frog Colostethus panamansis (Anura: Dendrobatidae). Metabolites. 2020; 10(10):406. https://doi.org/10.3390/metabo10100406

Chicago/Turabian Style

Martin H., Christian, Roberto Ibáñez, Louis-Félix Nothias, Andrés M. Caraballo-Rodríguez, Pieter C. Dorrestein, and Marcelino Gutiérrez. 2020. "Metabolites from Microbes Isolated from the Skin of the Panamanian Rocket Frog Colostethus panamansis (Anura: Dendrobatidae)" Metabolites 10, no. 10: 406. https://doi.org/10.3390/metabo10100406

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