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Accurate Quantification of Lipid Species by Electrospray Ionization Mass Spectrometry — Meets a Key Challenge in Lipidomics

by 1 and 2,*
1
Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA
2
Sanford-Burnham Medical Research Institute, Orlando, FL 32827, USA
*
Author to whom correspondence should be addressed.
Metabolites 2011, 1(1), 21-40; https://doi.org/10.3390/metabo1010021
Received: 8 October 2011 / Revised: 2 November 2011 / Accepted: 4 November 2011 / Published: 11 November 2011
(This article belongs to the Special Issue Lipidomics)
Electrospray ionization mass spectrometry (ESI-MS) has become one of the most popular and powerful technologies to identify and quantify individual lipid species in lipidomics. Meanwhile, quantitative analysis of lipid species by ESI-MS has also become a major obstacle to meet the challenges of lipidomics. Herein, we discuss the principles, advantages, and possible limitations of different mass spectrometry-based methodologies for lipid quantification, as well as a few practical issues important for accurate quantification of individual lipid species. Accordingly, accurate quantification of individual lipid species, one of the key challenges in lipidomics, can be practically met. View Full-Text
Keywords: electrospray ionization mass spectrometry; lipidomics; quantification; shotgun lipidomics electrospray ionization mass spectrometry; lipidomics; quantification; shotgun lipidomics
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MDPI and ACS Style

Yang, K.; Han, X. Accurate Quantification of Lipid Species by Electrospray Ionization Mass Spectrometry — Meets a Key Challenge in Lipidomics. Metabolites 2011, 1, 21-40. https://doi.org/10.3390/metabo1010021

AMA Style

Yang K, Han X. Accurate Quantification of Lipid Species by Electrospray Ionization Mass Spectrometry — Meets a Key Challenge in Lipidomics. Metabolites. 2011; 1(1):21-40. https://doi.org/10.3390/metabo1010021

Chicago/Turabian Style

Yang, Kui, and Xianlin Han. 2011. "Accurate Quantification of Lipid Species by Electrospray Ionization Mass Spectrometry — Meets a Key Challenge in Lipidomics" Metabolites 1, no. 1: 21-40. https://doi.org/10.3390/metabo1010021

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