Next Article in Journal
Synthesis and Evaluation of (1,4-Disubstituted)-1,2,3-triazoles as Estrogen Receptor Beta Agonists
Previous Article in Journal
Ruellia tuberosa Ethyl Acetate Leaf Extract Induces Apoptosis and Cell Cycle Arrest in Human Breast Cancer Cell Line, MCF-7
 
 
Article

Apoptosis Induction Associated with Enhanced ER Stress Response and Up-Regulation of c-Jun/p38 MAPK Proteins in Human Cervical Cancer Cells by Colocasia esculenta var. aquatilis Hassk Extract

Department of Biochemistry, Faculty of Medicine, Srinkharinwirot University, Bangkok 10110, Thailand
*
Author to whom correspondence should be addressed.
Academic Editor: Michael Danilenko
Sci. Pharm. 2022, 90(3), 45; https://doi.org/10.3390/scipharm90030045
Received: 27 May 2022 / Revised: 4 July 2022 / Accepted: 7 July 2022 / Published: 25 July 2022
Colocasia esculenta var. Aquatilis Hassk, elephant ear (CF-EE) has been widely used as traditional food and medicine. It also shows other therapeutic properties, such as antimicrobial and anti-cancer activity. In this study, we aim to investigate the effect of CF-EE extract on apoptosis induction associated with ER stress in cervical cancer HeLa cells. Cell viability was determined by MTT assay. Assessments of nuclear morphological changes, mitochondrial membrane potential, and intracellular reactive oxygen species (ROS) production were conducted by hoeshst33342, JC-1, and DCFH-DA fluorescence staining, respectively. Sub-G1 DNA content was analyzed by flow cytometry, and protein expression was determined by Western blotting. The results demonstrate that CF-EE extract suppressed HeLa cell growth and induced nuclear condensation and apoptotic bodies. There was also a loss of mitochondrial membrane potential and increased apoptosis marker protein expression, including Bax, cleaved-caspase-7, and cleaved-PARP. In addition, the results show that CF-EE extract induced ROS, increased ER stress proteins (GRP78 and CHOP), enhanced p38 and c-Jun phosphorylation, and inhibited Akt expression in HeLa cells. In summary, CF-EE extract induced apoptotic cell death-associated ROS-induced ER stress and the MAPK/AKT signaling pathway. Therefore, CF-EE extract has anticancer therapeutic potential for cervical cancer treatment in the future. View Full-Text
Keywords: Colocasia esculenta var. aquatilis Hassk; cervical cancer; endoplasmic reticulum stress; apoptosis; MAPK Colocasia esculenta var. aquatilis Hassk; cervical cancer; endoplasmic reticulum stress; apoptosis; MAPK
Show Figures

Figure 1

MDPI and ACS Style

Chomlamay, N.; Poorahong, W.; Innajak, S.; Watanapokasin, R. Apoptosis Induction Associated with Enhanced ER Stress Response and Up-Regulation of c-Jun/p38 MAPK Proteins in Human Cervical Cancer Cells by Colocasia esculenta var. aquatilis Hassk Extract. Sci. Pharm. 2022, 90, 45. https://doi.org/10.3390/scipharm90030045

AMA Style

Chomlamay N, Poorahong W, Innajak S, Watanapokasin R. Apoptosis Induction Associated with Enhanced ER Stress Response and Up-Regulation of c-Jun/p38 MAPK Proteins in Human Cervical Cancer Cells by Colocasia esculenta var. aquatilis Hassk Extract. Scientia Pharmaceutica. 2022; 90(3):45. https://doi.org/10.3390/scipharm90030045

Chicago/Turabian Style

Chomlamay, Natharika, Watcharaporn Poorahong, Sukanda Innajak, and Ramida Watanapokasin. 2022. "Apoptosis Induction Associated with Enhanced ER Stress Response and Up-Regulation of c-Jun/p38 MAPK Proteins in Human Cervical Cancer Cells by Colocasia esculenta var. aquatilis Hassk Extract" Scientia Pharmaceutica 90, no. 3: 45. https://doi.org/10.3390/scipharm90030045

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop