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Article

Concepts for New Rapid Simple HPLC Method for Quantification of Fosfomycin Trometamol in Pharmaceutical Dosage Forms with Direct UV Detection

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Replek Farm Ltd., 1000 Skopje, North Macedonia
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Department of Pharmaceutical Chemistry, I. Horbachevsky Ternopil National Medical University, 46001 Ternopil, Ukraine
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Department of Organic and Bioorganic Chemistry, Zaporizhzhia State Medical University, 69000 Zaporizhzhia, Ukraine
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Institute of Medicinal and Pharmaceutical Chemistry, Technische Universität Braunschweig, 38106 Braunschweig, Germany
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Natural and Medical Sciences Research Center, University of Nizwa, P.O. Box 33, Birkat Al Mauz, Nizwa 616, Oman
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Department of Psychiatry, Narcology and Medical Psychology, I. Horbachevsky Ternopil National Medical University, 46001 Ternopil, Ukraine
*
Author to whom correspondence should be addressed.
Academic Editor: Thierry Besson
Sci. Pharm. 2022, 90(2), 35; https://doi.org/10.3390/scipharm90020035
Received: 7 April 2022 / Revised: 8 May 2022 / Accepted: 17 May 2022 / Published: 24 May 2022
Two different concepts for developing direct HPLC-UV methods for quantifying fosfomycin trometamol were developed without any derivatization and modification of the analyte. In the first concept, without the use of alkylamines as ion-pairs in the mobile phase, by using cyanopropyl CN and a strong anion-exchanger column, we investigated the possibility of their highly polar and anion-exchanging forces and mechanisms to retain, separate and detect trometamol without the help of additional agents or modifiers. In the second concept, the most frequent reversed-phase C18 columns with different characteristics and vendors were tested in combination with different length-based alkylamines with 3–10 C atoms in their chains. In our research, we found that the ion-pairing of fosfomycin with 6–10 C-atom-based alkyl-length of aliphatic chains manifested the most appropriate strength of interactions between alkyl-paired trometamol molecules and octadecylsilane or C18 bonded RP column to achieve optimal retention, selectivity and peak shape on chromatograms, with the possibility for the fine-tuning of elution time. The simplicity of our method concept omits the need for expensive and sophisticated columns like HILIC, C30 graphite carbon, and mixed-mode-based columns for easier retaining, separation, and determination of fosfomycin, and for its quantification purposes, especially in high-throughput analyses in regular quality-control laboratories. View Full-Text
Keywords: amines; ion-pairing; HPLC; fosfomycin trometamol amines; ion-pairing; HPLC; fosfomycin trometamol
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MDPI and ACS Style

Piponski, M.; Stoimenova, T.B.; Melnyk, T.; Kovalenko, S.; Todevska, E.L.; Velkovski, M.; Deeb, S.E.; Mysula, Y.; Logoyda, L. Concepts for New Rapid Simple HPLC Method for Quantification of Fosfomycin Trometamol in Pharmaceutical Dosage Forms with Direct UV Detection. Sci. Pharm. 2022, 90, 35. https://doi.org/10.3390/scipharm90020035

AMA Style

Piponski M, Stoimenova TB, Melnyk T, Kovalenko S, Todevska EL, Velkovski M, Deeb SE, Mysula Y, Logoyda L. Concepts for New Rapid Simple HPLC Method for Quantification of Fosfomycin Trometamol in Pharmaceutical Dosage Forms with Direct UV Detection. Scientia Pharmaceutica. 2022; 90(2):35. https://doi.org/10.3390/scipharm90020035

Chicago/Turabian Style

Piponski, Marjan, Tanja B. Stoimenova, Tetiana Melnyk, Sergiy Kovalenko, Elena L. Todevska, Marjan Velkovski, Sami E. Deeb, Yuriy Mysula, and Liliya Logoyda. 2022. "Concepts for New Rapid Simple HPLC Method for Quantification of Fosfomycin Trometamol in Pharmaceutical Dosage Forms with Direct UV Detection" Scientia Pharmaceutica 90, no. 2: 35. https://doi.org/10.3390/scipharm90020035

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