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Sci. Pharm. 2019, 87(1), 2; https://doi.org/10.3390/scipharm87010002

Preliminary Phytochemical Analysis of Berberis goudotii Triana & Planch. ex Wedd. (Berberidaceae) with Anticariogenic and Antiperiodontal Activities

1
Department of Pharmacy, Faculty of Sciences, Universidad Nacional de Colombia, 111321 Bogotá, Colombia
2
Department of Chemistry, Faculty of Sciences, Pontificia Universidad Javeriana, 110231 Bogotá, Colombia
3
Dentistry Research Center, Faculty of Dentistry, Pontificia Universidad Javeriana, 110231 Bogotá, Colombia
4
Department of Microbiology, Faculty of Sciences, Pontificia Universidad Javeriana, 110231 Bogotá, Colombia
*
Authors to whom correspondence should be addressed.
Received: 17 November 2018 / Revised: 22 December 2018 / Accepted: 28 December 2018 / Published: 12 January 2019
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Abstract

Berberis goudotii is an endemic Colombian plant found in the paramo ecosystem. It has been used in food preparation and as a medicinal plant for diverse treatments. Additionally, it is used as a mouthwash to strengthen the gums and combat throat irritations and periodontitis. The present research evaluated Berberis goudotii aerial parts extract and fractions antimicrobial activities. Ultrasonic-assisted extraction was used to attain total ethanol-water extract. Solid-liquid fractionation was used to obtain hexane fraction. The residue was dispersed in water and liquid-liquid fractionation was carried-out to acquire dichloromethane, butanol and water fractions. Preliminary phytochemical analysis was performed on total extract and phenol, polyphenol, flavonoid, and proanthocyanidin, while tannin content was quantified. Antimicrobial activity assessment was performed by agar diffusion method using disks and wells employing Ceftazidime as a positive control against Streptococcus mutans, Streptococcus sobrinus, Lactobacillus acidophilus, Lactobacillus casei, Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum. Antimicrobial activity was determined as relative percentage inhibition (RPI), minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Phenols (92.5 ± 7.7 mg GA/10 g), polyphenols (87.7 ± 8.1 mg PG/10 g) and tannins (44.1 ± 4.3 mg PG/10 g) were among the highest secondary metabolites observed. Total extract presented an MBC of 1.0 µg/µL against cariogenic bacteria (Streptococcus mutans and Streptococcus sobrinus) and 0.12 µg/µL against bacteria associated with periodontal disease (Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum). Butanol and hexane fractions showed antiperiodontal activity with MBC of 0.12 and 1.0 µg/µL, respectively. In conclusion, Berberis goudotii total extract demonstrated antimicrobial activity against cariogenic and periodontal microorganisms, on the other hand, hexane and butanol fractions displayed antiperiodontal activity. View Full-Text
Keywords: Berberis goudotii; anticariogenic; antiperiodontal; Streptococcus mutans; Streptococcus sobrinus; Lactobacillus acidophilus; Lactobacillus casei; Porphyromonas gingivalis; Prevotella intermedia; Fusobacterium nucleatum; bacteriostatic; bactericide Berberis goudotii; anticariogenic; antiperiodontal; Streptococcus mutans; Streptococcus sobrinus; Lactobacillus acidophilus; Lactobacillus casei; Porphyromonas gingivalis; Prevotella intermedia; Fusobacterium nucleatum; bacteriostatic; bactericide
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Sequeda-Castañeda, L.G.; Muñoz-Realpe, C.C.; Celis-Zambrano, C.A.; Gutiérrez-Prieto, S.J.; Luengas-Caicedo, P.E.; Gamboa, F. Preliminary Phytochemical Analysis of Berberis goudotii Triana & Planch. ex Wedd. (Berberidaceae) with Anticariogenic and Antiperiodontal Activities. Sci. Pharm. 2019, 87, 2.

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