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Sci. Pharm. 2018, 86(4), 46; https://doi.org/10.3390/scipharm86040046

NO-Donor Nitrosyl Iron Complex with 2-Aminophenolyl Ligand Induces Apoptosis and Inhibits NF-κB Function in HeLa Cells

1
Institute of Problems of Chemical Physics RAS, 142432 Chernogolovka, Russia
2
Faculty of Fundamental Physical and Chemical Engineering, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia
3
Medicinal Chemistry Research and Education Center, Moscow Region State University, 141014 Mytishchi, Russia
*
Author to whom correspondence should be addressed.
Received: 5 September 2018 / Revised: 8 October 2018 / Accepted: 9 October 2018 / Published: 11 October 2018
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Abstract

NO donating iron nitrosyl complex with 2-aminothiophenyl ligand (2-AmPh complex) was studied for its ability to cause cell death and affect nuclear factor kappa B (NF-κB) signaling. The complex inhibited viability of HeLa cells and induced cell death that was accompanied by loss of mitochondrial membrane potential and characteristic for apoptosis phosphatidylserine externalization. At IC50, 2-AmPh caused decrease in nuclear content of NF-κB p65 polypeptide and mRNA expression of NF-κB target genes encoding interleukin-8 and anti-apoptotic protein BIRC3. mRNA levels of interleukin-6 and anti-apoptotic protein BIRC2 encoding genes were not affected. Our data demonstrate that NO donating iron nitrosyl complex 2-AmPh can inhibit tumor cell viability and induce apoptosis that is preceded by impairment of NF-κB function and suppression of a subset of NF-κB target genes. View Full-Text
Keywords: iron nitrosyl complex; 2-aminothiophenyl; apoptosis; NF-κB; p65; IL6; IL8; BIRC2; BIRC3 iron nitrosyl complex; 2-aminothiophenyl; apoptosis; NF-κB; p65; IL6; IL8; BIRC2; BIRC3
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Stupina, T.; Balakina, A.; Kondrat’eva, T.; Kozub, G.; Sanina, N.; Terent’ev, A. NO-Donor Nitrosyl Iron Complex with 2-Aminophenolyl Ligand Induces Apoptosis and Inhibits NF-κB Function in HeLa Cells. Sci. Pharm. 2018, 86, 46.

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