Leishmanicidal Activity of Biogenic Fe3O4 Nanoparticles
Mehrdad Khatami 1,2
, Hajar Alijani 1,*, Iraj Sharifi 3,*, Fatemeh Sharifi 4, Shahram Pourseyedi 5, Sam Kharazi 1, Marcos Augusto Lima Nobre 6 and Manouchehr Khatami 7
, Hajar Alijani 1,*, Iraj Sharifi 3,*, Fatemeh Sharifi 4, Shahram Pourseyedi 5, Sam Kharazi 1, Marcos Augusto Lima Nobre 6 and Manouchehr Khatami 7
1
School of Medicine, Bam University of Medical Sciences, Bam, Iran
2
Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
3
Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran
4
Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
5
Department of Biotechnology, Shahid Bahonar University of Kerman, Kerman, Iran
6
Fac de Ciências e Tecnologia-FCT, Universidade Estadual Paulista-UNESP, Presidente Prudente-SP 19060-900, Brazil
7
Department of Radiology, Kerman University of Medical Sciences, Kerman, Iran
*
Authors to whom correspondence should be addressed.
Academic Editor: Gernot A. Eller
Sci. Pharm. 2017, 85(4), 36; https://doi.org/10.3390/scipharm85040036
Received: 31 August 2017 / Revised: 6 November 2017 / Accepted: 7 November 2017 / Published: 20 November 2017
Abstract
Abstract: Due to the multiplicity of useful applications of metal oxide nanoparticles (ONPs) in medicine are growing exponentially, in this study, Fe3O4 (iron oxide) nanoparticles (IONPs) were biosynthesized using Rosemary to evaluate the leishmanicidal efficiency of green synthesized IONPs. This is the first report of the leishmanicidal efficiency of green synthesized IONPs against Leishmania major. The resulting biosynthesized IONPs were characterized by ultraviolet-visible spectroscopy (UV-Vis), X-ray diffraction (XRD), transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FTIR). The leishmanicidal activity of IONPS was studied via 3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results showed the fabrication of the spherical shape of monodisperse IONPs with a size 4 ± 2 nm. The UV-visible spectrophotometer absorption peak was at 334 nm. The leishmanicidal activity of biogenic iron oxide nanoparticles against Leishmania major (promastigote) was also studied. The IC50 of IONPs was 350 µg/mL. In this report, IONPs were synthesized via a green method. IONPs are mainly spherical and homogeneous, with an average size of about 4 nm, and were synthesized here using an eco-friendly, simple, and inexpensive method. View Full-TextKeywords:
biosynthesis; iron oxide nanoparticles; IONPs; leishmanicidal
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Khatami, M.; Alijani, H.; Sharifi, I.; Sharifi, F.; Pourseyedi, S.; Kharazi, S.; Lima Nobre, M.A.; Khatami, M. Leishmanicidal Activity of Biogenic Fe3O4 Nanoparticles. Sci. Pharm. 2017, 85, 36.
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