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Volume 77
Issue 1
10.3797/scipharm.0810-07
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Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2016). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
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Article

Viability of the Human Adenocarcinoma Cell Line Caco-2: Influence of Cryoprotectant, Freezing Rate, and Storage Temperature

by
Iris GÜLL
1,
Paula MARQUES ALVES
2,
Franz GABOR
1 and
Michael WIRTH
1,*
1
Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstraße 14, 1090, Vienna, Austria
2
ITQB-UNL/IBET, Apartado 12, 27801-901, Oeiras, Portugal
*
Author to whom correspondence should be addressed.
Sci. Pharm. 2009, 77(1), 133-142; https://doi.org/10.3797/scipharm.0810-07
Submission received: 10 October 2008 / Accepted: 8 January 2009 / Published: 9 January 2009
Versions Notes

Abstract

The freezing process as well as cryostorage may induce molecular and cellular changes due to osmotic stress. Currently, standard protocols for cryopreservation of mammalian cells recommend slow freezing and rapid thawing to avoid intracellular ice crystal formation and osmotic damage. As an appropriate freezing process should guarantee adequate cell viability, we evaluated the impact of two cryoprotectants, a commercially available cryopreservation medium, two freezing rates, as well as two storage temperatures on the viability of Caco-2 cells. The freezing parameters were optimized by carefully determining vitality, cell count, proliferation, and functional differentiation of the cells. Though at least 90% of the cells were viable after one freezing/thawing cycle, adequate recovery of proliferation and differentiation is obtained not until 10 days post thawing. Interestingly, addition of 10% PEG 200 as cryoprotectant yielded best results. All in all, our results should give valuable advice for the optimization of the cryopreservation protocol of individual cell lines.
Keywords: Caco-2; Cryopreservation; Freezing rate; Cryoprotectant; Viability Caco-2; Cryopreservation; Freezing rate; Cryoprotectant; Viability

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MDPI and ACS Style

GÜLL, I.; MARQUES ALVES, P.; GABOR, F.; WIRTH, M. Viability of the Human Adenocarcinoma Cell Line Caco-2: Influence of Cryoprotectant, Freezing Rate, and Storage Temperature. Sci. Pharm. 2009, 77, 133-142. https://doi.org/10.3797/scipharm.0810-07

AMA Style

GÜLL I, MARQUES ALVES P, GABOR F, WIRTH M. Viability of the Human Adenocarcinoma Cell Line Caco-2: Influence of Cryoprotectant, Freezing Rate, and Storage Temperature. Scientia Pharmaceutica. 2009; 77(1):133-142. https://doi.org/10.3797/scipharm.0810-07

Chicago/Turabian Style

GÜLL, Iris, Paula MARQUES ALVES, Franz GABOR, and Michael WIRTH. 2009. "Viability of the Human Adenocarcinoma Cell Line Caco-2: Influence of Cryoprotectant, Freezing Rate, and Storage Temperature" Scientia Pharmaceutica 77, no. 1: 133-142. https://doi.org/10.3797/scipharm.0810-07

APA Style

GÜLL, I., MARQUES ALVES, P., GABOR, F., & WIRTH, M. (2009). Viability of the Human Adenocarcinoma Cell Line Caco-2: Influence of Cryoprotectant, Freezing Rate, and Storage Temperature. Scientia Pharmaceutica, 77(1), 133-142. https://doi.org/10.3797/scipharm.0810-07

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