Next Article in Journal
Hepatitis C in Pregnancy
Previous Article in Journal
Levels of Soluble Endothelium Adhesion Molecules and Complications among Sickle Cell Disease Patients in Ghana
Open AccessFeature PaperCase Report

Use of Leukocyte Platelet (L-PRF) Rich Fibrin in Diabetic Foot Ulcer with Osteomyelitis (Three Clinical Cases Report)

1
Unit of Dermosurgery Cutaneous Transplantations and Hard-to-Heal Wound, “Villa Fiorita” Private Hospital, 81031 Aversa, CE, Italy
2
School of Medicine, University of Salerno Italy, 84084 Fisciano, SA, Italy
3
Institute for the Studies and Care of Diabetics, Abetaia, 81020 Casagiove, CE, Italy
4
Pathological Anatomy, “Pineta Grande” Private Hospital, 81030 Castelvolturno, CE, Italy
5
Faculty of Medicine and Surgery, Vasile Goldis Western University of Arad, 310025 Arad, Romania
*
Author to whom correspondence should be addressed.
Diseases 2018, 6(2), 30; https://doi.org/10.3390/diseases6020030
Received: 21 March 2018 / Revised: 18 April 2018 / Accepted: 21 April 2018 / Published: 24 April 2018
In this study, the use of fibrin rich in leukocytes and platelets (L-PRF) was explored to heal osteomyelitis ulcers in a diabetic foot. The goal was to standardize the utilization of L-PRF in patients with osteomyelitis to direct it for healing. L-PRF was obtained autologously from the peripheral blood of the diabetic patients (n = 3) having osteomyelitis and skin lesions for at least six months. The L-PRF and supernatant serum were inserted into the skin lesion to the bone after a surgical debridement. The evolution of lesions over time was analyzed. All three patients showed positivity to the Probe-to-Bone test and Nuclear Magnetic Resonance detected cortico-periosteal thickening and/or outbreaks of spongy cortical osteolysis in adjacency of the ulcer. The infections were caused by Cocci Gram-positive bacteria, such as S. Aureus, S. β-hemolytic, S. Viridans and Bacilli; and Gram-negative such as Pseudomonas, Proteus, Enterobacter; and yeast, Candida. The blood count did not show any significant alterations. To date, all three patients have healed skin lesions (in a patient for about two years) with no evidence of infection. These preliminary results showed that L-PRF membranes could be a new method of therapy in such problematic diseases. Overall, the L-PRF treatment in osteomyelitis of a diabetic foot seems to be easy and cost-effective by regenerative therapy of chronic skin lesions. In addition, it will improve our understanding of wound healing. View Full-Text
Keywords: osteomyelitis; buffy coat; growth factor level; platelet-rich fibrin; thrombocyte concentrate osteomyelitis; buffy coat; growth factor level; platelet-rich fibrin; thrombocyte concentrate
Show Figures

Figure 1

MDPI and ACS Style

Crisci, A.; Marotta, G.; Licito, A.; Serra, E.; Benincasa, G.; Crisci, M. Use of Leukocyte Platelet (L-PRF) Rich Fibrin in Diabetic Foot Ulcer with Osteomyelitis (Three Clinical Cases Report). Diseases 2018, 6, 30.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop